A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS
文献类型：期刊论文
| 作者 | Rebecca_Page@brown.edu; TWood@engr.psu.edu; Lord, DM; Cheng, HY; Osbourne, DO; Hong, SH; Sanchez-Torres, V; Quiroga, C; Zheng, K; Herrmann, T |
| 刊名 | NATURE CHEMICAL BIOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 8期号:10页码:855-861 |
| ISSN号 | 1552-4450 |
| 英文摘要 | Among bacterial toxin-antitoxin systems, to date no antitoxin has been identified that functions by cleaving toxin mRNA. Here we show that YjdO (renamed GhoT) is a membrane lytic peptide that causes ghost cell formation (lysed cells with damaged membranes) and increases persistence (persister cells are tolerant to antibiotics without undergoing genetic change). GhoT is part of a new toxin-antitoxin system with YjdK (renamed GhoS) because in vitro RNA degradation studies, quantitative real-time reverse-transcription PCR and whole-transcriptome studies revealed that GhoS masks GhoT toxicity by cleaving specifically yjdO (ghoT) mRNA. Alanine substitutions showed that Arg28 is important for GhoS activity, and RNA sequencing indicated that the GhoS cleavage site is rich in U and A. The NMR structure of GhoS indicates it is related to the CRISPR-associated-2 RNase, and GhoS is a monomer. Hence, GhoT-GhoS is to our knowledge the first type V toxin-antitoxin system where a protein antitoxin inhibits the toxin by cleaving specifically its mRNA. |
| 学科主题 | Biochemistry & Molecular Biology |
| 资助项目 | LMB |
| 语种 | 英语 |
| WOS记录号 | WOS:000309056200011 |
| 公开日期 | 2013-08-28 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/9796]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | Rebecca_Page@brown.edu; TWood@engr.psu.edu |
| 推荐引用方式 GB/T 7714 | Rebecca_Page@brown.edu,TWood@engr.psu.edu,Lord, DM,et al. A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS[J]. NATURE CHEMICAL BIOLOGY,2012,8(10):855-861. |
| APA | Rebecca_Page@brown.edu.,TWood@engr.psu.edu.,Lord, DM.,Cheng, HY.,Osbourne, DO.,...&Wood, TK.(2012).A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS.NATURE CHEMICAL BIOLOGY,8(10),855-861. |
| MLA | Rebecca_Page@brown.edu,et al."A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS".NATURE CHEMICAL BIOLOGY 8.10(2012):855-861. |
入库方式： OAI收割
来源：南海海洋研究所
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