Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides)
文献类型：期刊论文
| 作者 | Guo, ML; Ji, HS; Yan, Y; Ouyang, ZL; Huang, XH; Wei, JG; Hang, YH; Qin, QW; qinqw@scsio.ac.cn |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 38期号:1页码:108-116 |
| 关键词 | Thioredoxin Epinephelus Coioides Sgiv Intracellular Localization Antioxidant Capacity |
| ISSN号 | 0145-305X |
| 英文摘要 | Thioredoxins (TRXs) are a family of small, highly conserved proteins that are essential for the maintenance of cellular homeostasis. In this study, a thioredoxin gene was cloned from orange-spotted grouper, Epinephelus coioides (designated as Ec-TRX). The full-length cDNA of Ec-TRX was comprised of 767 bp with a 327 bp open reading frame that encodes a putative protein of 108 amino acids. Quantitative real-time PCR analysis revealed that the Ec-TRX mRNA was distributed abundantly in grouper, E. coioides skin and liver, and the expression in liver was up-regulated after viral challenge with Singapore grouper iridovirus (SGIV). Recombinant Ec-TRX (rEc-TRX) was expressed in Escherichia coli BL21 (DE3) and purified for mouse anti-Ec-TRX serum preparation. The rEc-TRX fusion protein was demonstrated to possess the expected redox activity in enzymatic analysis, and scavenge free radicals and protect supercoiled DNA from oxidative damage induced by a metal-ion catalyzed oxidation reaction. Subcellular localization revealed that Ec-TRX was distributed in both cytoplasm and nucleus. Overexpression of Ec-TRX in grouper spleen (GS) cells could promote the growth of GS cells and inhibit the replication of SGIV. These results suggest that Ec-TRX could function as an important antioxidant in a physiological context, and perhaps is involved in the responses to viral challenge. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology ; Zoology |
| 资助项目 | LMB |
| 语种 | 英语 |
| WOS记录号 | WOS:000308271100012 |
| 公开日期 | 2013-08-28 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/9826]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | qinqw@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Guo, ML,Ji, HS,Yan, Y,et al. Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,38(1):108-116. |
| APA | Guo, ML.,Ji, HS.,Yan, Y.,Ouyang, ZL.,Huang, XH.,...&qinqw@scsio.ac.cn.(2012).Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,38(1),108-116. |
| MLA | Guo, ML,et al."Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 38.1(2012):108-116. |
入库方式： OAI收割
来源：南海海洋研究所
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