Novel polymorphism of internal transcribed spacers (ITS) and their utilization in phylogenetic analysis of Neanthes glandicincta (Annelida: Polychaeta: Nereididae)
文献类型：期刊论文
| 作者 | Zhou, H; Gu, JG; Shen, PP; pshen@scsio.ac.cn |
| 刊名 | ECOTOXICOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 21期号:6页码:1717-1725 |
| 关键词 | Neanthes Glandicincta Internal Transcribed Spacers Variation Polymorphism Phylogenetic Analysis |
| ISSN号 | 0963-9292 |
| 英文摘要 | Sequences of internal transcribed spacers (ITS1 and ITS2) are increasingly being used to infer phylogenetic relationships at or below species levels. Here we report a novel case of ITS polymorphism within Neanthes glandicincta (Annelida: Polychaeta: Nereididae). Two types of ITS sequence (Type I and Type II) were cloned and sequenced, which showed significant differences both in nucleotide composition and length. Variations of these two types sequences also differed from each other with Type I was highly divergent while Type II was highly conserved. Phylogenetic trees inferred from ITS1 and ITS2 sequences showed striking discrepancy in N. glandicincta. Non-concerted evolution of multi-gene is suggested to be responsible for the high degree of polymorphism in ITS regions. Due to the two divergent types of ITS presented within a single N. glandicincta individual, the utilization of ITS regions for delineation of population or closely related species cannot be substantiated. The finding of different types of ITS in a single individual also stresses the need for analyzing a large number of clones whenever ITS sequences obtained by PCR amplification and cloning are being used in phylogenetic reconstruction. |
| 学科主题 | Ecology ; Environmental Sciences ; Toxicology |
| 资助项目 | LMB |
| 语种 | 英语 |
| WOS记录号 | WOS:000306592100016 |
| 公开日期 | 2013-08-28 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/9850]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | pshen@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Zhou, H,Gu, JG,Shen, PP,et al. Novel polymorphism of internal transcribed spacers (ITS) and their utilization in phylogenetic analysis of Neanthes glandicincta (Annelida: Polychaeta: Nereididae)[J]. ECOTOXICOLOGY,2012,21(6):1717-1725. |
| APA | Zhou, H,Gu, JG,Shen, PP,&pshen@scsio.ac.cn.(2012).Novel polymorphism of internal transcribed spacers (ITS) and their utilization in phylogenetic analysis of Neanthes glandicincta (Annelida: Polychaeta: Nereididae).ECOTOXICOLOGY,21(6),1717-1725. |
| MLA | Zhou, H,et al."Novel polymorphism of internal transcribed spacers (ITS) and their utilization in phylogenetic analysis of Neanthes glandicincta (Annelida: Polychaeta: Nereididae)".ECOTOXICOLOGY 21.6(2012):1717-1725. |
入库方式： OAI收割
来源：南海海洋研究所
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