Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides
文献类型：期刊论文
| 作者 | Wei, SN; Huang, YH; Cai, J; Huang, XH; Fu, J; Qin, QW; qinqw@scsio.ac.cn |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 33期号:2页码:186-196 |
| 关键词 | Epinephelus Coioides C-type Lysozyme Antimicrobial Antivirus |
| ISSN号 | 1050-4648 |
| 英文摘要 | Lysozymes are key proteins of the host innate immune system against pathogen infection. In this study, a c-type lysozyme gene (Ec-lysC) was cloned and characterized from orange-spotted grouper, Epinephelus coioides. The full-length Ec-lysC cDNA is composed of 533 bp and encodes a polypeptide of 144-residue protein with 94% identity to lysC of Kelp grouper, Epinephelus bruneus. The genomic DNA of Ec-lysC consists of 4 exons and 3 introns, with a total length of 1897 bp. Amino acid sequence alignment showed that Ec-lysC possessed conserved catalytic residues (Glu50 and Asp67) and "GSTDYGIFQINS" motif. RT-PCR results showed that Ec-lysC transcript was most abundant in head kidney and less in muscle. The expression of Ec-lysC was differentially up-regulated in head kidney after stimulation with lipopolysaccharide (LPS), Vibrio alginolyticus and Singapore grouper iridovirus (SGIV). Subcellular localization analysis revealed that Ec-lysC was distributed predominantly in the cytoplasm. The recombinant Ec-lysC (rEc-lysC) had lytic activities against Gram-positive bacteria Micrococcus lysodeikticus, Staphylococcus aureus, Streptococcus iniae and Gram-negative bacteria V. alginolyticus. The lysozyme acted on M. lysodeikticus cell walls as shown by scanning electron microscopy (SEM). Furthermore, overexpression of Ec-lysC in grouper cells delayed the occurrence of CPE induced by SGIV and inhibited the viral gene transcription significantly. Taken together, Ec-lysC might play an important role in grouper innate immune responses to invasion of bacterial and viral pathogens. C-type lysozyme gene from E. coioides (Ec-lysC) was identified and characterized. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 资助项目 | LMB |
| 语种 | 英语 |
| WOS记录号 | WOS:000306632800004 |
| 公开日期 | 2013-08-28 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/9852]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | qinqw@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Wei, SN,Huang, YH,Cai, J,et al. Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(2):186-196. |
| APA | Wei, SN.,Huang, YH.,Cai, J.,Huang, XH.,Fu, J.,...&qinqw@scsio.ac.cn.(2012).Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,33(2),186-196. |
| MLA | Wei, SN,et al."Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 33.2(2012):186-196. |
入库方式： OAI收割
来源：南海海洋研究所
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