Detection and characterization of two insertion sequences in Vibrio alginolyticus
文献类型：期刊论文
| 作者 | cqhu@scsio.ac.cn; Ren, CH; Jiang, X; Sun, HY; Luo, P; Chen, C; Zhao, Z; Hu, CQ |
| 刊名 | ANNALS OF MICROBIOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 62期号:1页码:69-75 |
| 关键词 | Insertion Sequences Vibrio Alginolyticus Detection Characterization |
| ISSN号 | 1590-4261 |
| 英文摘要 | Vibrio alginolyticus is the dominant Vibrio species and an opportunistic pathogen for marine animals and human. It has been reported to be an important reservoir for virulence genes in aquatic environment. In this study, a total of 187 isolates of V. alginolyticus from fish ponds or coral reefs in the South China Sea were examined for the presence of two insertion sequences, ISVal-1 and ISVal-2. In addition, the characteristics of the two ISs were described. The results showed that 58.82% of the examined isolates contained ISVal-1 and 23.53% had ISVal-2. For both ISVal-1 and ISVal-2, the detection rate for ISs from the fish ponds was lower than that from the coral reef. Sequence analysis revealed that ISVal-1 had the typical features of the IS5 family which contained 19 bp terminal inverted repeats (IRs) and a large open reading frame containing a conserved DDE motif. ISVal-2 possessed genetic structures identical to the IS3 family which had imperfect IRs and two partially overlapping reading frames, OrfA and orfB. A putative helix-turn-helix (HTH) motif, a presumed leucine zipper (LZ) motif, and a conserved DDE motif were contained in the two Orfs. In addition, the data suggested that ISVal-1 and ISVal-2 in V. alginolyticus may be horizontally transferred from other Vibrio species and play certain roles in the virulence of the pathogen. To our knowledge, this is the first report on ISs in V. alginolyticus. |
| 学科主题 | Biotechnology & Applied Microbiology ; Microbiology |
| 资助项目 | LMB |
| 语种 | 英语 |
| WOS记录号 | WOS:000300605800010 |
| 公开日期 | 2013-08-28 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/9968]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | cqhu@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | cqhu@scsio.ac.cn,Ren, CH,Jiang, X,et al. Detection and characterization of two insertion sequences in Vibrio alginolyticus[J]. ANNALS OF MICROBIOLOGY,2012,62(1):69-75. |
| APA | cqhu@scsio.ac.cn.,Ren, CH.,Jiang, X.,Sun, HY.,Luo, P.,...&Hu, CQ.(2012).Detection and characterization of two insertion sequences in Vibrio alginolyticus.ANNALS OF MICROBIOLOGY,62(1),69-75. |
| MLA | cqhu@scsio.ac.cn,et al."Detection and characterization of two insertion sequences in Vibrio alginolyticus".ANNALS OF MICROBIOLOGY 62.1(2012):69-75. |
入库方式： OAI收割
来源：南海海洋研究所
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