Characterization of role of the toxR gene in the physiology and pathogenicity of Vibrio alginolyticus
文献类型：期刊论文
| 作者 | Chen, C; Wang, QB; Liu, ZH; Zhao, JJ; Jiang, X; Sun, HY; Ren, CH; Hu, CQ; cqhu@scsio.ac.cn |
| 刊名 | ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 101期号:2页码:281-288 |
| 关键词 | Vibrio Alginolyticus Toxr Bile Resistance Outer-membrane Protein Pathogenicity |
| ISSN号 | 0003-6072 |
| 英文摘要 | toxR, a conserved virulence-associated gene in vibrios, is identified in Vibrio alginolyticus ZJ51-O, a pathogenic strain isolated from diseased fish. To reveal the role of ToxR in the pathogenicity of V. alginolyticus, a deletion mutant was constructed by allelic exchange. The mutant showed the same level of growth in trypticase soy broth (TSB) and iron-limiting condition, as the wild type strain. However, deletion of toxR severely reduced resistance against bile salts and the capability of biofilm formation. Outer-membrane protein (OMP) analysis showed that a 37-kD protein was absent and a 43-kD protein was decreased in the mutant. By MS/MS, the two proteins are identified as the homologues of OmpT and OmpN, respectively. These data suggest that ToxR might have enhanced the bile resistance and biofilm formation through modulating the production of OMP without affecting the ability of iron acquisition and the virulence to the fish via injection. These results indicate that ToxR may assist V. alginolyticus to colonize on the surface of the fish intestine which is crucial for the initiation of the infection, though it may not be involved in the proliferation of the bacteria in the host tissue. |
| 学科主题 | Microbiology |
| 资助项目 | LMB |
| 语种 | 英语 |
| WOS记录号 | WOS:000299293900007 |
| 公开日期 | 2013-08-28 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/9984]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 通讯作者 | cqhu@scsio.ac.cn |
| 推荐引用方式 GB/T 7714 | Chen, C,Wang, QB,Liu, ZH,et al. Characterization of role of the toxR gene in the physiology and pathogenicity of Vibrio alginolyticus[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2012,101(2):281-288. |
| APA | Chen, C.,Wang, QB.,Liu, ZH.,Zhao, JJ.,Jiang, X.,...&cqhu@scsio.ac.cn.(2012).Characterization of role of the toxR gene in the physiology and pathogenicity of Vibrio alginolyticus.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,101(2),281-288. |
| MLA | Chen, C,et al."Characterization of role of the toxR gene in the physiology and pathogenicity of Vibrio alginolyticus".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 101.2(2012):281-288. |
入库方式： OAI收割
来源：南海海洋研究所
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