Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis
文献类型：期刊论文
| 作者 | Li, M; wangjg@ihb.ac.cn; Li, AH; Wang, JG; Wu, HB; Wu, YS; Zhang, JY |
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2005 |
| 卷号 | 64期号:2页码:121-126 |
| 关键词 | Glugea Epinephelusis Microsporidia Epinephelus Akaara Humoral Immune Responses Antigenicity |
| ISSN号 | 0177-5103 |
| 英文摘要 | The humoral immune responses of grouper Epinephelus akaara to a natural infection with Glugea epinephelusis was studied by ELISA utilizing intact mature spores as the coated antigen. Results showed that a specific humoral immune response was elicited, but the intensity of infection (in terms of the number of cysts) was not related to the antibody level in naturally infected hosts. The differences in the antigenicity of intact mature spores and soluble spore proteins derived from cracked mature spores were also analyzed. Results suggested that similar antigen epitopes existed between the 2 groups. Additionally, antigen component patterns and the distribution of antigen with immunogenicity were investigated by using the western blot and the immunofluorescent antibody technique (IFAT). The new parasitic microsporidium has specific polypeptide patterns comparable to the reported fish microsporidians. The main antigenic substances are concentrated on the surface of spores, and are mostly located on the anterior and posterior end of the spore bodies. Most surface components of the G. epinephelusis spores are soluble, The potential role of the surface components in initiating infection was also discussed. |
| 学科主题 | Fisheries ; Veterinary Sciences |
| 资助项目 | 广东省海洋药物重点实验室 |
| WOS记录号 | WOS:000230065600004 |
| 公开日期 | 2011-06-29 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/2243]  |
| 专题 | 南海海洋研究所_广东省海洋药物重点实验室 |
| 通讯作者 | wangjg@ihb.ac.cn |
| 推荐引用方式 GB/T 7714 | Li, M,wangjg@ihb.ac.cn,Li, AH,et al. Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis[J]. DISEASES OF AQUATIC ORGANISMS,2005,64(2):121-126. |
| APA | Li, M.,wangjg@ihb.ac.cn.,Li, AH.,Wang, JG.,Wu, HB.,...&Zhang, JY.(2005).Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis.DISEASES OF AQUATIC ORGANISMS,64(2),121-126. |
| MLA | Li, M,et al."Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis".DISEASES OF AQUATIC ORGANISMS 64.2(2005):121-126. |
入库方式： OAI收割
来源：南海海洋研究所
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