Isolation and puritication of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Chen, F; Li, HB; sfchen@hkusua.hku.hk |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2005 |
| 卷号 | 1074期号:3页码:107-110 |
| 关键词 | Scutellaria Baicalensis Counter-current Chromatography Preparative Chromatography Plant Materials Baicalein Wogonin Oroxylin a |
| ISSN号 | 0021-9673 |
| 英文摘要 | The medicinal plant Scutellaria baicatensis Georgi has been used widely in traditional Chinese medicine for anti-inflammation, anticancer, antiviral and antibacterial infections, reducing the total cholesterol level and decreasing blood pressures. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of three bioactive flavonoids, namely, baicalein, wogonin and oroxylin A, from S. baicalensis Georgi. Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-n-butanol-water (1: 1: 8: 10, v/v/v/v) was successfully performed by increasing the flow-rate of the mobile phase stepwise from 1.0 to 2.0 ml min(-1) after 4 h. The components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 144.8 mg of baicalein at 95.7% purity, 50.2 mg of wogonin at 98.5% purity, and 12.4 mg of oroxylin A at 93.2% purity from 500 mg of the crude extract in a one-step separation. The recoveries of baicalein, wogonin and oroxylin A were 92.7%, 91.6% and 92.5%, respectively. (c) 2005 Published by Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemical Research Methods ; Chemistry, Analytical |
| 资助项目 | 广东省海洋药物重点实验室 |
| WOS记录号 | WOS:000229192500014 |
| 公开日期 | 2011-06-29 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/2247]  |
| 专题 | 南海海洋研究所_广东省海洋药物重点实验室 |
| 通讯作者 | sfchen@hkusua.hku.hk |
| 推荐引用方式 GB/T 7714 | Chen, F,Li, HB,sfchen@hkusua.hku.hk. Isolation and puritication of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1074(3):107-110. |
| APA | Chen, F,Li, HB,&sfchen@hkusua.hku.hk.(2005).Isolation and puritication of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,1074(3),107-110. |
| MLA | Chen, F,et al."Isolation and puritication of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 1074.3(2005):107-110. |
入库方式： OAI收割
来源：南海海洋研究所
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