Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current chromatography coupled with reversed-phase high-performance liquid chromatography
文献类型：期刊论文
| 作者 | wwujun2003@yahoo.com; Huang, K; Long, LJ; Song, Y; Wu, J; Lei, L; Long, BW |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2006 |
| 卷号 | 386期号:40732页码:2169-2174 |
| ISSN号 | 1618-2642 |
| 关键词 | Amphidinium Carterae High-speed Counter-current Chromatography Reversed-phase High-performance Liquid Chromatography Preparative Chromatography Chlorophyll a |
| 英文摘要 | In our research into chlorophylls of marine dinoflagellates, chlorophyll a was separated rapidly from the hexane extract of Amphidinium carterae in three steps. The first step was silica gel column chromatography, where elution was performed with 0-50% ethyl acetate in n-hexane. The second was high-speed counter-current chromatography using a two-phase solvent system consisting of n-hexane-ethyl acetate-methanol-water (5:5:5:1, v/v), and the third step was preparative reversed-phase high-performance liquid chromatography using a solvent system of acetone-water (89:11, v/v). HPLC analysis showed that the purity of chlorophyll a from the second step was over 83%, and after the third it was over 99%. Thirty milligrams of chlorophyll a was isolated from a crude sample of 250 mg of chlorophylls, and its structure was identified by analyzing its MS, H-1 NMR and C-13 NMR spectra. |
| 学科主题 | Biochemical Research Methods ; Chemistry, Analytical |
| URL标识 | 查看原文 |
| 资助项目 | 广东省海洋药物重点实验室 |
| WOS记录号 | WOS:000242144200027 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5168]  |
| 专题 | 南海海洋研究所_广东省海洋药物重点实验室 |
| 通讯作者 | wwujun2003@yahoo.com |
| 推荐引用方式 GB/T 7714 | wwujun2003@yahoo.com,Huang, K,Long, LJ,et al. Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current chromatography coupled with reversed-phase high-performance liquid chromatography[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,386(40732):2169-2174. |
| APA | wwujun2003@yahoo.com.,Huang, K.,Long, LJ.,Song, Y.,Wu, J.,...&Long, BW.(2006).Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current chromatography coupled with reversed-phase high-performance liquid chromatography.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,386(40732),2169-2174. |
| MLA | wwujun2003@yahoo.com,et al."Development of an efficient method for the preparative isolation and purification of chlorophyll a from a marine dinoflagellate Amphidinium carterae by high-speed counter-current chromatography coupled with reversed-phase high-performance liquid chromatography".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 386.40732(2006):2169-2174. |
入库方式： OAI收割
来源：南海海洋研究所
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