Development of an immobilization and detection method of Enterobacter sakazakii from powdered infant formula
文献类型：期刊论文
| 作者 | Yang, XJ; Zhou, YH; Wu, QP; Xu, XK; Ye, YW; Zhang, JM; mylovelyswallow@yeah.net; wuqp203@yahoo.com.cn |
| 刊名 | FOOD MICROBIOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 25期号:5页码:648-652 |
| 关键词 | Enterobacter Sakazakii Immobilization Duplex Pcr Powdered Infant Formula |
| ISSN号 | 0740-0020 |
| 英文摘要 | Enterobacter sakazakii is an emerging opportunistic pathogen that is associated with rare but life-threatening cases of meningitis, necrotizing enterocolitis, and sepsis in premature and full-term infants. In the present study, a procedure was developed for immobilization of E. sakazakii with zirconium hydroxide coupled with detection by a species-specific duplex PCR, based on 16s-23s rDNA internal transcribed spacer (ITS) and ompA gene. Specificity of duplex PCR was tested against two-type strains, six isolates of E. sakazakii and other eight non-E. sakazakii species. When pure culture of E. sakazakii was used for immobilization, total recovery rate ranged from 79.4% to 99.6% of input bacteria, and the detection limit of duplex PCR was 3 x 10(5) CFU/ml. Different levels of E. sakazakii were inoculated into 90 ml reconstituted powdered infant formula (PIF), and detection limit of duplex PCR was 3 x 10(0) CFU/ ml with 24-30h enrichment after immobilization. When the experiment was performed in the presence of 10(2) CFU/ml Salmonella typhimurium, the detection limit of duplex PCR was not affected after enrichment. Seven out of 13 commercial PIF were detected positive by duplex PCR after immobilization, while only three were positive by biological methods. This study demonstrates that the combination of immobilization method with duplex PCR is easy, rapid, and efficient, and may have applications for the detection of E. sakazakii in more PIF samples. (C) 2008 Elsevier Ltd. All rights reserved. |
| 学科主题 | Biotechnology & Applied Microbiology ; Food Science & Technology ; Microbiology |
| URL标识 | 查看原文 |
| 资助项目 | 海洋生物 |
| WOS记录号 | WOS:000257628700003 |
| 公开日期 | 2011-07-03 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/5354]  |
| 专题 | 南海海洋研究所_海洋生物 |
| 通讯作者 | mylovelyswallow@yeah.net; wuqp203@yahoo.com.cn |
| 推荐引用方式 GB/T 7714 | Yang, XJ,Zhou, YH,Wu, QP,et al. Development of an immobilization and detection method of Enterobacter sakazakii from powdered infant formula[J]. FOOD MICROBIOLOGY,2008,25(5):648-652. |
| APA | Yang, XJ.,Zhou, YH.,Wu, QP.,Xu, XK.,Ye, YW.,...&wuqp203@yahoo.com.cn.(2008).Development of an immobilization and detection method of Enterobacter sakazakii from powdered infant formula.FOOD MICROBIOLOGY,25(5),648-652. |
| MLA | Yang, XJ,et al."Development of an immobilization and detection method of Enterobacter sakazakii from powdered infant formula".FOOD MICROBIOLOGY 25.5(2008):648-652. |
入库方式： OAI收割
来源：南海海洋研究所
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