Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties
文献类型:期刊论文
作者 | Wang, Yonggang1,2; Li, Yuanli1; Li, Shaowei3; Li, QingYang4; Fan, Wenguang2; Kiatoukosin, Lormoua2; Chen, Jixiang1,5 |
刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES |
出版日期 | 2019-07-15 |
卷号 | 133页码:172-183 |
ISSN号 | 0141-8130 |
关键词 | Endophytic fungus exopolysaccharide Production optimization and purification Structure and bioactivities |
DOI | 10.1016/j.ijbiomac.2019.03.246 |
通讯作者 | Wang, Yonggang(412316788@163.com) ; Chen, Jixiang(chenjixiang@lut.cn) |
英文摘要 | The yield, composition, structure and biofunction of microorganism exopolysaccharide (EPS) depended on their genetics and metabolic pathways. An EPS-producing endophytic fungus was isolated from Angelica sinensis planted in Dingxi, Gansu, China, and identified as Alternaria tenuissima Fl. The production optimization, purification and characterization of the purified EPS from A. tenuissima Fl and the in-vitro antioxidant potentials was investigated. Results showed the optimal medium composition were 8% mannose, 2% yeast extract, 0.04% MgSO4. 7H20, 0.005% Vc, and the optimal cultivation conditions were set at initial pH 7.0, culture temperature 30 C, inoculum size of 3%, 180 r/min for 5 d. The EPS purified by ion exchange column chromatography and gel chromatography was a non-reducing sugar and glycoprotein with pyranoid ring by Fourier transform infrared spectroscopy (FT-IR) analysis, displayed a porous network structure by scanning electron microscope (SEM) observation and exhibited a high thermal stability with the degradation temperature of 303.9 C by thermogravimetric analysis (TG/DSC). Molecular weight of the purified EPS was 3.246 x 104 g/mol, and EPS was composed of o-galacturonic acid, rhamnose, o-mannose, glucose, and o-galactose in ratio of 0.45:3.02:3.25:1.0:0.95. The EPS exhibited strong scavenging activity and may be a new source of natural antioxidant. (C) 2019 Elsevier B.V. All rights reserved. |
WOS关键词 | STRUCTURAL-CHARACTERIZATION ; EXOPOLYSACCHARIDE PRODUCTION ; OPTIMIZATION ; CULTURE ; ANTITUMOR ; EXTRACTION ; PULLULAN |
资助项目 | National Natural Science Foundation of China[31760028] ; National Natural Science Foundation of China[31460032] ; Youth Talent Support Program of Lanzhou University of Technology[2018] |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000472685700021 |
资助机构 | National Natural Science Foundation of China ; Youth Talent Support Program of Lanzhou University of Technology |
源URL | [http://ir.igsnrr.ac.cn/handle/311030/58404] |
专题 | 中国科学院地理科学与资源研究所 |
通讯作者 | Wang, Yonggang; Chen, Jixiang |
作者单位 | 1.Lanzhou Univ Technol, Sch Energy & Power Engn, Lanzhou 730050, Gansu, Peoples R China 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China 3.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China 4.Cent S Univ, Xiangya Sch Med, Changsha 410000, Hunan, Peoples R China 5.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Gansu, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Yonggang,Li, Yuanli,Li, Shaowei,et al. Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,133:172-183. |
APA | Wang, Yonggang.,Li, Yuanli.,Li, Shaowei.,Li, QingYang.,Fan, Wenguang.,...&Chen, Jixiang.(2019).Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,133,172-183. |
MLA | Wang, Yonggang,et al."Extracellular polysaccharides of endophytic fungus Alternaria tenuissima F1 from Angelica sinensis: Production conditions, purification, and antioxidant properties".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 133(2019):172-183. |
入库方式: OAI收割
来源:地理科学与资源研究所
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