Spectroscopy studies of interaction between methylene blue and herring sperm DNA
文献类型：期刊论文
| 作者 | Fei, D ; Wang, XM ; Li, HB ; Ding, LS ; Hu, YM ; Zhang, H ; Zhao, SL |
| 刊名 | ACTA CHIMICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 66期号:4页码:443_448 |
| 关键词 | methylene blue acridine orange herring sperm DNA action mode |
| ISSN号 | 0567-7351 |
| 其他题名 | 亚甲基蓝与鲱鱼精DNA相互作用的光谱法研究 |
| 产权排序 | 2 |
| 通讯作者 | Fei, D, SW Univ Sci & Technol, Sch Mat Sci & Engn, Dept Chem, Mianyang 621010, Peoples R China. |
| 中文摘要 | The UV-VIS spectroscopy and fluorescence spectroscopy were applied to investigate the mechanism of the interaction between methylene blue (MB) and herring sperm DNA by using acridine orange as a spectral probe in Tris-HCl buffer (pH 7.0). The chief action mode was comfirmed to be an intercalation binding at a lower MB concentration but the mixed action modes at a higher concentration. Combining ratio of the binary complex is n(MB) : n(DNA)= 10 : 1, the binding constant of MB with DNA is K-26 degrees C(circle minus)=2.46 X 10(5) L center dot mol(-1) and the apparent molor absorptivity is epsilon = 5.70 X 10(6) L center dot mol(-1)center dot cm(-1). Meanwhile, the interaction of MB with DNA was effected by the changes of pH and temperature. The results deduce that the interaction between MB and DNA is an enthalpy driven reaction. |
| 学科主题 | Chemistry, Multidisciplinary |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000254396700008 |
| 公开日期 | 2011-07-08 |
| 源URL | [http://210.75.237.14/handle/351003/18129]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Fei, D,Wang, XM,Li, HB,et al. Spectroscopy studies of interaction between methylene blue and herring sperm DNA[J]. ACTA CHIMICA SINICA,2008,66(4):443_448. |
| APA | Fei, D.,Wang, XM.,Li, HB.,Ding, LS.,Hu, YM.,...&Zhao, SL.(2008).Spectroscopy studies of interaction between methylene blue and herring sperm DNA.ACTA CHIMICA SINICA,66(4),443_448. |
| MLA | Fei, D,et al."Spectroscopy studies of interaction between methylene blue and herring sperm DNA".ACTA CHIMICA SINICA 66.4(2008):443_448. |
入库方式： OAI收割
来源：成都生物研究所
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