Study on the interaction between Riboflavin and herring sperm DNA by fluorescence spectrometry
文献类型：期刊论文
| 作者 | Hu, YM ; Wang, XM ; Fei, D ; Dong, FQ ; Ding, LS |
| 刊名 | ACTA CHIMICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 66期号:10页码:1245_1251 |
| 关键词 | Riboflavin acridine orange herring sperm DNA action mode |
| ISSN号 | 0567-7351 |
| 其他题名 | 荧光光谱法研究核黄素与鲱鱼精DNA的相互作用 |
| 产权排序 | 2 |
| 通讯作者 | Hu, YM, SW Univ Sci & Technol, Dept Chem, Mianyang 621010, Peoples R China. |
| 中文摘要 | The interaction of Riboflavin with DNA was studied in a Britton-Robinson buffer solution by employing acridine orange as a fluorescence probe. The fluorescence spectra indicated that a kind of compound of Riboflavin and DNA was formed when pH was 7.25. The binary compound ratio is n(RF) : n(DNA)= 5 : 1; the binding constant of Riboflavin with DNA compound is 2.53 x 10(6) L/mol at a temperature of 28 degrees C; and the thermodynamic parameters of the interaction by a double reciprocal method were calculated: Delta H-r(m)circle minus= -6.06 x 10(4) J/mol, Delta S-r(m)circle minus= -78.70 J/(mol.K), Delta(r)G(m)(circle minus)= -3.69x10(4) J/mol at 28 degrees C. The interaction of Riboflavin with DNA was studied also through methods of viscosity and Scatchard as well as salt effect. The results confirm that the groove binding and electrostatic binding are the two major modes of the interaction between Riboflavin and herring sperm DNA. |
| 学科主题 | Chemistry, Multidisciplinary |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000256549500017 |
| 公开日期 | 2011-07-08 |
| 源URL | [http://210.75.237.14/handle/351003/18131]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Hu, YM,Wang, XM,Fei, D,et al. Study on the interaction between Riboflavin and herring sperm DNA by fluorescence spectrometry[J]. ACTA CHIMICA SINICA,2008,66(10):1245_1251. |
| APA | Hu, YM,Wang, XM,Fei, D,Dong, FQ,&Ding, LS.(2008).Study on the interaction between Riboflavin and herring sperm DNA by fluorescence spectrometry.ACTA CHIMICA SINICA,66(10),1245_1251. |
| MLA | Hu, YM,et al."Study on the interaction between Riboflavin and herring sperm DNA by fluorescence spectrometry".ACTA CHIMICA SINICA 66.10(2008):1245_1251. |
入库方式： OAI收割
来源：成都生物研究所
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