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An experimental design approach using response surface techniques to obtain optimal liquid chromatography and mass spectrometry conditions to determine the alkaloids in Meconopsi species
文献类型：期刊论文
| 作者 | Zhou, Y ; Song, JZ ; Choi, FFK ; Wu, HF ; Qiao, CF ; Ding, LS ; Gesang, SL ; Xu, HX |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2009 |
| 卷号 | 1216期号:42页码:7013_7023 |
| 关键词 | Response surface methodology (RSM) UHPLC-QTOF-ESI-MS Box-Behnken designs (BBD) Alkaloids Meconopsis species |
| ISSN号 | 0021-9673 |
| 产权排序 | 2 |
| 通讯作者 | Xu, HX, Hong Kong Jockey Club Inst Chinese Med, Shatin, Hong Kong, Peoples R China. |
| 中文摘要 | A statistic approach using response surface methodology (RSM) for optimization of the ultra-high performance liquid chromatography (UHPLC) gradient and ionization response of electrospray ionization mass spectrometry (ESI-MS) to analyze the main alkaloids from the plant matrices of six Meconopsi species is presented. The optimization was performed with Box-Behnken designs (BBD) and the multicriteria response variables were described using global Derringer's desirability. Four parameters of UHPLC and six major parameters of ESI-MS were investigated for their contribution to analytes separation and response, leading to a total of 27 and 54 experiments being performed for each instrument. respectively. Quantitative analysis of four main alkaloids in nine samples from six Meconopsis species was employed to evaluate the statistical significance of the parameters on UHPLC-QTOF/ESI-MS analytes response. The results indicated that the optimized UHPLC-QTOF-MS method is very sensitive with the limit of detections (LODs) ranging from 0.5 to 0.1 ng/ml. The overall intra-day and the inter-clay variations were less than 2.45%. The recovery of the method was in the range of 94.3-104.8% with RSD less than 4.0%. This approach has important implication in sensitivity enhancement of the ultra-trace determination of alkaloids from complex matrixes in the fields of natural products, metabolomics and pharmacokinetics. (C) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemical Research Methods; Chemistry, Analytical |
| 收录类别 | SCI |
| 资助信息 | Hong Kong Jockey Club Charities Trust Fund ; National Key Technology R&D Program of China [2007BAI31B02] |
| 语种 | 英语 |
| WOS记录号 | WOS:000270750900006 |
| 公开日期 | 2011-07-08 |
| 源URL | [http://210.75.237.14/handle/351003/18213]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Zhou, Y,Song, JZ,Choi, FFK,et al. An experimental design approach using response surface techniques to obtain optimal liquid chromatography and mass spectrometry conditions to determine the alkaloids in Meconopsi species[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(42):7013_7023. |
| APA | Zhou, Y.,Song, JZ.,Choi, FFK.,Wu, HF.,Qiao, CF.,...&Xu, HX.(2009).An experimental design approach using response surface techniques to obtain optimal liquid chromatography and mass spectrometry conditions to determine the alkaloids in Meconopsi species.JOURNAL OF CHROMATOGRAPHY A,1216(42),7013_7023. |
| MLA | Zhou, Y,et al."An experimental design approach using response surface techniques to obtain optimal liquid chromatography and mass spectrometry conditions to determine the alkaloids in Meconopsi species".JOURNAL OF CHROMATOGRAPHY A 1216.42(2009):7013_7023. |
入库方式： OAI收割
来源：成都生物研究所
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