Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
文献类型：期刊论文
| 作者 | Zhou, Y ; Xu, G ; Choi, FFK ; Ding, LS ; Bin Han, Q ; Song, JZ ; Qiao, CF ; Zhao, QS ; Xu, HX |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2009 |
| 卷号 | 1216期号:24页码:4847_4858 |
| 关键词 | Salvia species UHPLC-QTOF-MS/MS Diterpenoids Fragmentation pathway Quantification Principal component analysis |
| ISSN号 | 0021-9673 |
| 产权排序 | 3 |
| 通讯作者 | Zhao, QS, Chinese Acad Sci, Kunming Inst Bot, State Key LPhytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China. |
| 中文摘要 | An on-line ultra-high-performance liquid chromatography (UHPLC) coupled with photodiode-array detection and quadrupole time-of-flight tandem mass spectrometry (QTOF-MS/MS) method has been optimized and established for the qualitative and quantitative analysis of the important diterpenoids in the methanol extracts of 12 Salvia species. Specific marker components were identified for the classification of the Salvia samples by principal component analysis. The accurate mass measurement within 3 ppm error for all the protonated molecules and Subsequent fragment ions offers higher quality structural information for interpretation of fragmentation pathways of various groups of diterpenoids. Thus. a total of 21 diterpenoids from different Salvio species were separated within 10 min, and were unequivocally or tentatively identified via comparisons with authentic standards and literature. This UHPLC-QTOF-MS/MS method was validated to be sensitive, precise and accurate with the limit of detections at 3.0-16 ng/ml. and the overall intra-day and the inter-clay variations less than 3%. The recovery of the method was in the range of 96.2-101.8%, with relative standard deviation (RSD) less than 3.0%. The results demonstrated that the qualitative and quantitative differences in diterpenoids were not only useful for chemotaxonomy in some Salvia species but also for the standardization and differentiation of large numbers of similar samples. (C) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemical Research Methods; Chemistry, Analytical |
| 收录类别 | SCI |
| 资助信息 | Hong Kong Jockey Club Charities Trust Fund ; National Natural Science Foundation of China [20702054]; Natural Science Foundation of Yunnan Province [2006CO044Q] |
| 语种 | 英语 |
| WOS记录号 | WOS:000266671400005 |
| 公开日期 | 2011-07-08 |
| 源URL | [http://210.75.237.14/handle/351003/18267]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Zhou, Y,Xu, G,Choi, FFK,et al. Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(24):4847_4858. |
| APA | Zhou, Y.,Xu, G.,Choi, FFK.,Ding, LS.,Bin Han, Q.,...&Xu, HX.(2009).Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1216(24),4847_4858. |
| MLA | Zhou, Y,et al."Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1216.24(2009):4847_4858. |
入库方式： OAI收割
来源：成都生物研究所
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