Ligand fishing from Dioscorea nipponica extract using human serum albumin functionalized magnetic nanoparticles
文献类型：期刊论文
| 作者 | Qing, Lin-Sen ; Xue, Ying ; Zheng, Yi ; Xiong, Jing ; Liao, Xun ; Ding, Li-Sheng ; Li, Bo-Gang ; Liu, Yi-Ming |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2010 |
| 卷号 | 1217期号:28页码:4663-4668 |
| 关键词 | Ligand fishing Magnetic nanoparticles Human serum album Dioscorea nipponica Saponins Dioscin |
| ISSN号 | 0021-9673 |
| 产权排序 | 1 |
| 通讯作者 | Liao, X, Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China |
| 中文摘要 | Dioscorea nipponica and the preparations made from it have been used for long to prevent and treat coronary heart disease in traditional Chinese medicine. A group of steroidal saponins present in the plant are believed to be the active ingredients. It has been a challenge to study the individual saponins separately due to the similarities in their chemical and physical properties. In this work, human serum albumin (HSA) functionalized magnetic nanoparticles (MNPs) were used to isolate and identify saponin ligands that bind to HSA from D. nipponica extract. Electrospray ionization mass spectrometry (ESI-MS) was used for compound identification and semi-quantification. Three saponins, i.e. dioscin, gracillin, and pseudo-protodioscin showed affinity to HSA-MNPs and thus isolated effectively from the extract. The other two saponins detected in the extract (i.e. protodioscin and 26-O-beta-D-glucopyranosyl-3 beta,20 alpha,26-triol-25(R)-Delta(5,22)-dienofurostan-3-O-alpha-L-rhamnopyranosyl (1 -> 2)-[alpha-L-rhamnopyranosyl (1 -> 4)]-beta-D-glucopyranoside) exhibited no affinity at all. Among the three saponins fished out, dioscin bound to HSA much stronger than gracillin and pseudo-protodioscin did. The results indicated that affinity interaction between HSA immobilized on MNPs and small molecule compounds were highly dependent on chemical structures and, potentially, medicinal usefulness. The present work demonstrates a facile and effective way to isolate and identify ligands of receptors from medicinal plants. (C) 2010 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemical Research Methods; Chemistry, Analytical |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [20872137/B020402]; US National Institutes of Health [SC1GM089557] |
| 语种 | 英语 |
| WOS记录号 | WOS:000279378900001 |
| 公开日期 | 2011-07-08 |
| 源URL | [http://210.75.237.14/handle/351003/18411]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Qing, Lin-Sen,Xue, Ying,Zheng, Yi,et al. Ligand fishing from Dioscorea nipponica extract using human serum albumin functionalized magnetic nanoparticles[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(28):4663-4668. |
| APA | Qing, Lin-Sen.,Xue, Ying.,Zheng, Yi.,Xiong, Jing.,Liao, Xun.,...&Liu, Yi-Ming.(2010).Ligand fishing from Dioscorea nipponica extract using human serum albumin functionalized magnetic nanoparticles.JOURNAL OF CHROMATOGRAPHY A,1217(28),4663-4668. |
| MLA | Qing, Lin-Sen,et al."Ligand fishing from Dioscorea nipponica extract using human serum albumin functionalized magnetic nanoparticles".JOURNAL OF CHROMATOGRAPHY A 1217.28(2010):4663-4668. |
入库方式： OAI收割
来源：成都生物研究所
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