Qualitative and quantitative analysis of polycyclic polyprenylated acylphloroglucinols from Garcinia species using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
文献类型：期刊论文
| 作者 | Zhou, Yan ; Lee, Stephanie ; Choi, Franky Fung Kei ; Xu, Gang ; Liu, Xin ; Song, Jing-Zheng ; Li, Song-Lin ; Qiao, Chun-Feng ; Xu, Hong-Xi |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2010 |
| 卷号 | 678期号:1页码:96-107 |
| 关键词 | Polycyclic polyprenylated acylphloroglucinols (PPAPs) Garcinia species Ultra performance liquid chromatography Quadrupole time-of-flight mass spectrometry |
| ISSN号 | 0003-2670 |
| 产权排序 | 2 |
| 通讯作者 | Xu, HX, Hong Kong Jockey Club Inst Chinese Med, Bioinformat Ctr, Unit 703, 7th Flr,2 Sci Pk,Hong Kong Sci Pk, Shatin, Hong Kong, Peoples R China |
| 中文摘要 | Polycyclic polyprenylated acylphloroglucinols (PPAPs) are a group of natural products isolated from different Garcinia species with a wide range of important biological activities. In this study, an ultra performance liquid chromatography (UPLC) coupled to photodiode-array detection and quadrupole time-of-flight mass spectrometry (Q-TOF) method was developed to characterize 16 PPAPs in 10 Garcinia species. In source dissociation techniques based on cone voltage fragmentation were used to fragment the deprotonated molecules and multiple mass spectrometry (MS/MS) using ramping collision energy were used to further break down the resulting product ions. The resulting characteristic fragment ions were generated by cleavage of C1-C5 bond and C7-C8 bond through concerted pericyclic reaction, which is especially valuable for differentiating three types of PPAPs isomers. As such, two new PPAPs isomers present in minor amount in the extracts of Garcinia oblongifolia were tentatively characterized by comparing their tandem mass spectra to the known ones. In addition, an UPLC-Q-TOF-MS method was validated for the quantitative determination of PPAPs. The method exhibited limits of detection from 2.7 to 21.4 ng mL(-1) and intra-day and inter-day variations were less than 3.7% and the recovery was in the range of 89-107% with RSD less than 9.0%. This UPLC-Q-TOF-MS method has successfully been applied to quantify 16 PPAPs in 32 samples of 10 Garcinia species, which were found to be a rich source of PPAPs. (C) 2010 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry, Analytical |
| 收录类别 | SCI |
| 资助信息 | Hong Kong Jockey Club Charities Trust Fund |
| 语种 | 英语 |
| WOS记录号 | WOS:000282850800014 |
| 公开日期 | 2011-07-08 |
| 源URL | [http://210.75.237.14/handle/351003/18423]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Zhou, Yan,Lee, Stephanie,Choi, Franky Fung Kei,et al. Qualitative and quantitative analysis of polycyclic polyprenylated acylphloroglucinols from Garcinia species using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2010,678(1):96-107. |
| APA | Zhou, Yan.,Lee, Stephanie.,Choi, Franky Fung Kei.,Xu, Gang.,Liu, Xin.,...&Xu, Hong-Xi.(2010).Qualitative and quantitative analysis of polycyclic polyprenylated acylphloroglucinols from Garcinia species using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.ANALYTICA CHIMICA ACTA,678(1),96-107. |
| MLA | Zhou, Yan,et al."Qualitative and quantitative analysis of polycyclic polyprenylated acylphloroglucinols from Garcinia species using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry".ANALYTICA CHIMICA ACTA 678.1(2010):96-107. |
入库方式： OAI收割
来源：成都生物研究所
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