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The synergistic effects of multivalent ligand display and nanotopography on osteogenic differentiation of rat bone marrow stem cells
文献类型：期刊论文
| 作者 | Kaur, Gagandeep ; Wang, Chao ; Sun, Jian ; Wang, Qian |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2010 |
| 卷号 | 31期号:22页码:5813-5824 |
| 关键词 | Nanoparticle Nanotopography Bone marrow stromal cells Mesenchymal stem cell Virus substrate Osteogenic differentiation |
| ISSN号 | 0142-9612 |
| 产权排序 | 2 |
| 通讯作者 | Wang, QA, Univ S Carolina, Dept Chem & Biochem, 631 Sumter St, Columbia, SC 29208 USA |
| 中文摘要 | Cell substrate interactions play a vital role in governing crucial cell functions such as adhesion, proliferation and differentiation. Surface topography and chemical properties can initiate signaling cascades modulating cell behavior. However, mimicking extracellular environment to direct cell functions through cell surface interactions is challenging. In this report, we employed tobacco mosaic virus (TMV) as a model system to present nanotopographic features along with multivalent ligand display to study osteogenic differentiation of bone marrow stem cells (BMSCs). TMV is a rod shaped plant virus which is 300 nm in length and 18 nm in diameter. A single TMV rod comprises of 2130 identical coat proteins which assemble into the rod-like helical structure around the single strand of RNA. For the present study TMV was chemically modified with phosphate to induce calcium incorporation. Gene regulation during BMSC differentiation on TMV and TMV-phosphate (TMV-Phos) was studied over time points of 7, 14 and 21 days. We examined changes in gene expression of osteospecific genes (osteocalcin, osteopontin and runx2) which indicate that nanofeatures functionalized with phosphate groups exhibited significantly higher up regulation of osteospecific genes. Furthermore, we studied the gene regulation by coating Ti substrates with TMV and TMV-Phos. TMV-Phos coated substrates displayed higher expression of the studied genes as compared to Ti substrates. Our results imply that the differentiation capacity of BMSCs can be significantly enhanced through simple multivalent interactions with simple functional units rather than using complex biomolecules. (C) 2010 Elsevier Ltd. All rights reserved. |
| 学科主题 | Engineering, Biomedical; Materials Science, Biomaterials |
| 收录类别 | SCI |
| 资助信息 | US NSF [DMR-0706431, CHE-0748690]; US ARO [W911NF-09-1-0236]; Alfred P. Sloan Foundation ; Camille Dreyfus Teacher-Scholarship ; W. M. Keck Foundation |
| 语种 | 英语 |
| WOS记录号 | WOS:000279092900011 |
| 公开日期 | 2011-07-08 |
| 源URL | [http://210.75.237.14/handle/351003/18441]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Kaur, Gagandeep,Wang, Chao,Sun, Jian,et al. The synergistic effects of multivalent ligand display and nanotopography on osteogenic differentiation of rat bone marrow stem cells[J]. BIOMATERIALS,2010,31(22):5813-5824. |
| APA | Kaur, Gagandeep,Wang, Chao,Sun, Jian,&Wang, Qian.(2010).The synergistic effects of multivalent ligand display and nanotopography on osteogenic differentiation of rat bone marrow stem cells.BIOMATERIALS,31(22),5813-5824. |
| MLA | Kaur, Gagandeep,et al."The synergistic effects of multivalent ligand display and nanotopography on osteogenic differentiation of rat bone marrow stem cells".BIOMATERIALS 31.22(2010):5813-5824. |
入库方式： OAI收割
来源：成都生物研究所
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