Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China
文献类型：期刊论文
| 作者 | Bai, Yan1; Huang, Xiying1; Zhou, Xiangrui1; Xiang, Quanju1; Zhao, Ke1; Yu, Xiumei1; Chen, Qiang1; Jiang, Hao2 ; Nyima, Tashi3; Gao, Xue3
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| 刊名 | PEERJ
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| 出版日期 | 2019-08-14 |
| 卷号 | 7页码:22 |
| 关键词 | Hailuogou Glacier retreat area Primary succession Denitrifying bacteria Community Abundance |
| ISSN号 | 2167-8359 |
| DOI | 10.7717/peerj.7356 |
| 通讯作者 | Gu, Yunfu(guyf@sicau.edu.cn) |
| 英文摘要 | Background. The Hailuogou Glacier is located at the Gongga Mountain on the southeastern edge of the Tibetan Plateau, and has retreated continuously as a result of global warming. The retreat of the Hailuogou Glacier has left behind a primary succession along soil chronosequences. Hailuogou Glacier's retreated area provides an excellent living environment for the colonization of microbes and plants, making it an ideal model to explore plant successions, microbial communities, and the interaction of plants and microbes during the colonization process. However, to date, the density of the nitrogen cycling microbial communities remain unknown, especially for denitrifiers in the primary succession of the Hailuogou Glacier. Therefore, we investigated the structural succession and its driving factors for denitrifying bacterial communities during the four successional stages (0, 20, 40, and 60 years). Methods. The diversity, community composition, and abundance of nosZ-denitrifiers were determined using molecular tools, including terminal restriction fragment length polymorphism and quantitative polymerase chain reactions (qPCR). Results. nosZ-denitrifiers were more abundant and diverse in soils from successional years 20-60 compared to 0-5 years, and was highest in Site3 (40 years). The denitrifying bacterial community composition was more complex in older soils (40-60 years) than in younger soils (<= 20 years). The terminal restriction fragments (T-RFs) of Azospirillum (90 bp) and Rubrivivax (95 bp) were dominant in soisl during early successional stages (0-20 years) and in the mature phase (40-60 years), respectively. Specific T-RFs of Bradyrhizobium (100 bp) and Pseudomonas (275 bp) were detected only in Site3 and Site4, respectively. Moreover, the unidentified 175 bp T-RFs was detected only in Site3. Of the abiotic factors that were measured in this study, soil available phosphorus, available potassium and denitrifying enzyme activity (DEA) correlated significantly with the community composition of nosZ-denitrifiers (P < 0.05 by Monte Carlo permutation test within RDA analysis). |
| WOS关键词 | NITROUS-OXIDE REDUCTASE ; MICROBIAL COMMUNITY ; SOIL DEVELOPMENT ; GONGGA MOUNTAIN ; NUTRIENT LIMITATION ; NOSZ GENES ; ABUNDANCE ; CHRONOSEQUENCE ; FERTILIZATION ; WATER |
| 资助项目 | Science and Technology Department of the Tibet and Sichuan Agricultural University |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000480819100006 |
| 出版者 | PEERJ INC |
| 资助机构 | Science and Technology Department of the Tibet and Sichuan Agricultural University |
| 源URL | [http://ir.imde.ac.cn/handle/131551/26967]  |
| 专题 | 成都山地灾害与环境研究所_山地表生过程与生态调控重点实验室 |
| 通讯作者 | Gu, Yunfu |
| 作者单位 | 1.Sichuan Agr Univ, Coll Resources, Dept Microbiol, Chengdu, Sichuan, Peoples R China 2.Chinese Acad Sci, Inst Mt Hazards & Environm, Chengdu, Sichuan, Peoples R China 3.Tibet Acad Agr & Anim Husb Sci, Inst Agr Resources & Environm Sci, Lhasa, Tibet, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Yan,Huang, Xiying,Zhou, Xiangrui,et al. Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China[J]. PEERJ,2019,7:22. |
| APA | Bai, Yan.,Huang, Xiying.,Zhou, Xiangrui.,Xiang, Quanju.,Zhao, Ke.,...&Gu, Yunfu.(2019).Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China.PEERJ,7,22. |
| MLA | Bai, Yan,et al."Variation in denitrifying bacterial communities along a primary succession in the Hailuogou Glacier retreat area, China".PEERJ 7(2019):22. |
入库方式： OAI收割
来源：成都山地灾害与环境研究所
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