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  4. 中国科学院地球与行星物理重点实验室
Phylogenetic and Structural Identification of a Novel Magnetotactic Deltaproteobacteria Strain, WYHR-1, from a Freshwater Lake

文献类型：期刊论文

作者Li, Jinhua1,2,3; Zhang, Heng1,3; Liu, Peiyu1,3; Menguy, Nicolas4; Roberts, Andrew P.5; Chen, Haitao1,3; Wang, Yinzhao1,3; Pan, Yongxin1,3,6
刊名APPLIED AND ENVIRONMENTAL MICROBIOLOGY
出版日期2019-07-01
卷号85期号:14页码:11
关键词bacterial diversity bullet-shaped magnetosomes coupled fluorescence
ISSN号0099-2240
DOI10.1128/AEM.00731-19
英文摘要Magnetotactic bacteria (MTB) are phylogenetically diverse prokaryotes that are able to biomineralize intracellular, magnetic chains of magnetite or greigite nanocrystals called magnetosomes. Simultaneous characterization of MTB phylogeny and biomineralization is crucial but challenging because most MTB are extremely difficult to culture. We identify a large rod, bean-like MTB (tentatively named WYHR-1) from freshwater sediments of Weiyang Lake, Xi'an, China, using a coupled fluorescence and scanning electron microscopy approach at the single-cell scale. Phylogenetic analysis of 16S rRNA gene sequences indicates that WYHR-1 is a novel genus from the Deltaproteobacteria class. Transmission electron microscope observations reveal that WYHR-1 cells contain tens of magnetite magnetosomes that are organized into a single chain bundle along the cell long axis. Mature WYHR-1 magnetosomes are bullet-shaped, straight, and elongated along the [001] direction, with a large flat end terminated by a {100} face at the base and a conical top. This crystal morphology is distinctively different from bullet-shaped magnetosomes produced by other MTB in the Deltaproteobacteria class and the Nitrospirae phylum. This indicates that WYHR-1 may have a different crystal growth process and mechanism from other species, which results from species-specific magnetosome biomineralization in MTB. IMPORTANCE Magnetotactic bacteria (MTB) represent a model system for understanding biomineralization and are also studied intensively in biogeomagnetic and paleomagnetic research. However, many uncultured MTB strains have not been identified phylogenetically or investigated structurally at the single-cell level, which limits comprehensive understanding of MTB diversity and their role in biomineralization. We have identified a novel MTB strain, WYHR-1, from a freshwater lake using a coupled fluorescence and scanning electron microscopy approach at the single-cell scale. Our analyses further indicate that strain WYHR-1 represents a novel genus from the Deltaproteobacteria class. In contrast to bullet-shaped magnetosomes produced by other MTB in the Deltaproteobacteria class and the Nitrospirae phylum, WYHR-1 magnetosomes are bullet-shaped, straight, and highly elongated along the [001] direction, are terminated by a large {100} face at their base, and have a conical top. Our findings imply that, consistent with phylogenetic diversity of MTB, bullet-shaped magnetosomes have diverse crystal habits and growth patterns.
WOS关键词BACTERIA REVEALS ; MAGNETITE ; DIVERSITY ; BIOMINERALIZATION ; CRYSTALS ; GREIGITE ; PROKARYOTES ; NITROSPIRAE ; SIGNATURE ; EVOLUTION
资助项目National Natural Science Foundation of China[41890843] ; National Natural Science Foundation of China[41522402] ; National Natural Science Foundation of China[41621004] ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology[MGQNLM201704] ; Australian Research Council[DP140104544] ; Region Ile-de-France grant[SESAME 2000 E 1435]
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
语种英语
WOS记录号WOS:000476468600022
出版者AMER SOC MICROBIOLOGY
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology ; Australian Research Council ; Australian Research Council ; Region Ile-de-France grant ; Region Ile-de-France grant
源URL[http://ir.iggcas.ac.cn/handle/132A11/92928]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Li, Jinhua
作者单位1.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Geol, Qingdao, Shandong, Peoples R China
3.CNRS CAS, Int Associated Lab Evolut & Dev Magnetotact Multi, Beijing, Peoples R China
4.Sorbonne Univ, UPMC, MNHN, IMPMC,CNRS UMR 7590,IRD UMR 206, Paris, France
5.Australian Natl Univ, Res Sch Earth Sci, Canberra, ACT, Australia
6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Li, Jinhua,Zhang, Heng,Liu, Peiyu,et al. Phylogenetic and Structural Identification of a Novel Magnetotactic Deltaproteobacteria Strain, WYHR-1, from a Freshwater Lake[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2019,85(14):11.
APA Li, Jinhua.,Zhang, Heng.,Liu, Peiyu.,Menguy, Nicolas.,Roberts, Andrew P..,...&Pan, Yongxin.(2019).Phylogenetic and Structural Identification of a Novel Magnetotactic Deltaproteobacteria Strain, WYHR-1, from a Freshwater Lake.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,85(14),11.
MLA Li, Jinhua,et al."Phylogenetic and Structural Identification of a Novel Magnetotactic Deltaproteobacteria Strain, WYHR-1, from a Freshwater Lake".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 85.14(2019):11.

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来源：地质与地球物理研究所

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