Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes
文献类型：期刊论文
| 作者 | Hong,Pei1,2; Wu,Xingqiang1,2; Shu,Yilin3,4; Wang,ChunBo1,2; Tian,Cuicui1,2; Gong,Shihao1,2; Cai,Pei1,2; Donde,Oscar Omondi1,2; Xiao,Bangding1,2 |
| 刊名 | AMB Express
 |
| 出版日期 | 2019-08-19 |
| 卷号 | 9期号:1 |
| ISSN号 | 2191-0855 |
| 关键词 | Response surface methodology (RSM) DO concentration Denitrifier Lake surface sediment Denitrification traits |
| DOI | 10.1186/s13568-019-0855-9 |
| 通讯作者 | Xiao,Bangding(bdxiao618@163.com) |
| 英文摘要 | AbstractThe responses of denitrifiers and denitrification ability to dissolved oxygen (DO) concent in different layers of surface lake sediments are still poorly understood. Here, the optimal denitrification condition was constructed based on response surface methodology (RSM) to analyze the denitrification characteristics of surface sediments. The aerobic zone (AEZ), hypoxic zone (HYZ), up-anoxic zone (ANZ-1) and sub-anoxic zone (ANZ-2) were partitioned based on the oxygen contents, and sediments were collected using a customized-designed sub-millimeter scale sampling device. Integrated real-time quantitative PCR, Illumina Miseq-based sequencing and denitrifying enzyme activities analysis revealed that denitrification characteristics varied among different DO layers. Among the four layers, the DNA abundance and RNA expression levels of norB, nirS and nosZ were the highest at the aerobic layer, hypoxic layer and up-axoic layer, respectively. The hypoxia and up-anaerobic layer were the active nitrogen removal layers, since these two layers displayed the highest DNA abundance, RNA expression level and enzyme activities of denitrification functional genes. The abundance of major denitrifying bacteria showed significant differences among layers, with Azoarcus, Pseudogulbenkiania and Rhizobium identified as the main nirS, nirK and nosZ-based denitrifiers. Pearson’s correlation revealed that the response of denitrifiers to environmental factors differed greatly among DO layers. Furthermore, napA showed higher DNA abundance and RNA expression level in the aerobic and hypoxic layers than anaerobic layers, indicating that aerobic denitrifiers might play important roles at these layers. |
| 语种 | 英语 |
| 出版者 | Springer Berlin Heidelberg |
| WOS记录号 | BMC:10.1186/S13568-019-0855-9 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/33161]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 通讯作者 | Xiao,Bangding |
| 作者单位 | 1. 2. 3. 4. |
| 推荐引用方式 GB/T 7714 | Hong,Pei,Wu,Xingqiang,Shu,Yilin,et al. Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes[J]. AMB Express,2019,9(1). |
| APA | Hong,Pei.,Wu,Xingqiang.,Shu,Yilin.,Wang,ChunBo.,Tian,Cuicui.,...&Xiao,Bangding.(2019).Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes.AMB Express,9(1). |
| MLA | Hong,Pei,et al."Denitrification characterization of dissolved oxygen microprofiles in lake surface sediment through analyzing abundance, expression, community composition and enzymatic activities of denitrifier functional genes".AMB Express 9.1(2019). |
入库方式： OAI收割
来源：水生生物研究所
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