A label-free colorimetric detection of microRNA via hG-quadruplex-based signal quenching strategy
文献类型：期刊论文
| 作者 | Lan, Ling; Wang, Rui-Li; Liu, Li; Cheng, Liang |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2019-11-04 |
| 卷号 | 1079页码:207-211 |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2019.06.063 |
| 英文摘要 | Signal amplification strategy have been the intensive direction for highly-sensitive microRNA (miRNA) detection, however, it is worth noting that signal quenching approach is rarely realized. Inspired by the unwinding of G-quadruplex with Klenow Fragment polymerase (KF), a simple and label-free signal quenching system for detection of miRNAs was developed. In the presence of target miRNA, miRNA was used as a primer to promote KF activity resulting in the disruption of G-quadruplex structure of probe and the biological catalysis inactivation of DNAzyme formed by G-quadruplex structure and hemin. As a result, the probe would be transformed from G-quadruplex to duplex and the obvious differential signal could be observed in most cases even with naked eyes. With the significant signal decreasing, the target miRNA can be rapidly analyzed by UVevis spectra with a considerably low detection limit (4.5 nM). This method exhibits excellent selectivity and anti-interference ability by discriminating base-mismatched and other miRNA families from target miRNA. Meanwhile, a good recovery (90.7%similar to 102.4%) in 5% human serum was obtained and this strategy verified a high expression level of miR21 in total RNA of MCF-7 compared with 293T and HeLa. It implies that this assay is of great potential to be applied in biochemical research and clinical diagnosis. (C) 2019 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000478681100021 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/82574]  |
| 专题 | 中国科学院化学研究所 |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lan, Ling,Wang, Rui-Li,Liu, Li,et al. A label-free colorimetric detection of microRNA via hG-quadruplex-based signal quenching strategy[J]. ANALYTICA CHIMICA ACTA,2019,1079:207-211. |
| APA | Lan, Ling,Wang, Rui-Li,Liu, Li,&Cheng, Liang.(2019).A label-free colorimetric detection of microRNA via hG-quadruplex-based signal quenching strategy.ANALYTICA CHIMICA ACTA,1079,207-211. |
| MLA | Lan, Ling,et al."A label-free colorimetric detection of microRNA via hG-quadruplex-based signal quenching strategy".ANALYTICA CHIMICA ACTA 1079(2019):207-211. |
入库方式： OAI收割
来源：化学研究所
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