Linkage and sequence analysis of neutral oligosaccharides by negative-ion MALDI tandem mass spectrometry with laser-induced dissociation
文献类型：期刊论文
| 作者 | Huang, Chuncui; Yan, Jingyu; Zhan, Lingpeng; Zhao, Min; Zhou, Jinyu; Gao, Huanyu; Xie, Wenchun; Li, Yan; Chai, Wengang |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2019-09-13 |
| 卷号 | 1071页码:25-35 |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2019.04.067 |
| 英文摘要 | Mass spectrometry (MS) has become the primary method for high-sensitivity structural determination of oligosaccharides. Fragmentation in the negative-ion MS can provide a wealth of structural information and these can be used for sequence determination. However, although negative-ion MS of neutral oligosaccharide using the deprotonated molecule [M-H](-) as the precursor has been very successful for electrospray ionization (ESI), it has only limited success for matrix-assisted laser desorption/ionization (MALDI). In the present study, the features of negative-ion MALDI primary spectra were investigated in detail and the product-ion spectra using [M-H](-) and [M+Cl](-) as the precursors were carefully compared. The formation of [M-H](- )was the main difficulty for MALDI while [M+Cl](-) was proved to be useful as alternative precursor anion for MALDI-MS/MS to produce similar fragmentation for sequencing of neutral oligosaccharides. N-(1-naphthyl)ethylenediamine dihydrochloride was then used as both the matrix and the Cl- dopant to evaluate the extent of structural information that can be obtained by negative-ion fragmentation from [M+Cl](-) using laser-induced dissociation (LID)-MS/MS for linkage assignment of gluco-oligosaccharides and for typing of blood-group ABO(H) and Lewis antigens on either type 1 or type 2 backbone-chains. (C) 2019 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000468581400004 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/82595]  |
| 专题 | 中国科学院化学研究所 |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Chuncui,Yan, Jingyu,Zhan, Lingpeng,et al. Linkage and sequence analysis of neutral oligosaccharides by negative-ion MALDI tandem mass spectrometry with laser-induced dissociation[J]. ANALYTICA CHIMICA ACTA,2019,1071:25-35. |
| APA | Huang, Chuncui.,Yan, Jingyu.,Zhan, Lingpeng.,Zhao, Min.,Zhou, Jinyu.,...&Chai, Wengang.(2019).Linkage and sequence analysis of neutral oligosaccharides by negative-ion MALDI tandem mass spectrometry with laser-induced dissociation.ANALYTICA CHIMICA ACTA,1071,25-35. |
| MLA | Huang, Chuncui,et al."Linkage and sequence analysis of neutral oligosaccharides by negative-ion MALDI tandem mass spectrometry with laser-induced dissociation".ANALYTICA CHIMICA ACTA 1071(2019):25-35. |
入库方式： OAI收割
来源：化学研究所
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