Mono-fullerenols modulating cell stiffness by perturbing actin bundling
文献类型：期刊论文
| 作者 | Chen, K ; Qin, YX; Zhao, XY; Xia, SB; Zhang, JX; Lei RH(雷润宏); Chang YN(常亚男); Ceng L(曾立); Li J(李娟); Xing GM(邢更妹)
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| 刊名 | NANOSCALE
 |
| 出版日期 | 2018 |
| 卷号 | 10期号:4页码:1750-1758 |
| ISSN号 | 2040-3364 |
| DOI | 10.1039/c7nr07231g |
| 文献子类 | Article |
| 英文摘要 | Understanding what modulates the cell stiffness is important given its potential application as a diagnostic and medical target. Here, we investigated why and how mono-fullerenols affect the cell stiffness. We confirmed the fullerenol-modulation of cell stiffness using atomic force microscopy (AFM) with sphere tips and ascertained that the particles reduce the cell polarity. The structures of b-actin and f-actin were evaluated by inverted fluorescence microscopy, synchrotron radiation small angle X-ray scattering (SAXS), transmission electron microscopy (TEM) and AFM. Statistical and quantitative analyses of the SAXS data of fullerenol-treated b-actin and f-actin reveal a transformation from large-size to small-size b-actin and simultaneously to f-actin. The slight increase in f-actin diameter in the treated group suggests that fullerenols attach to the actin surface. We verified the attachment using AFM and high-resolution probes. Collectively, our results suggest that fullerenols hamper the bundling of f-actin to form b-actin by adhering to the surface of f-actin, weakening the bundle-based cell stiffness. |
| 电子版国际标准刊号 | 2040-3372 |
| WOS关键词 | BREAST-CANCER CELLS ; F-ACTIN ; STRUCTURAL FEATURES ; GOLD NANOPARTICLES ; LIPID-MEMBRANES ; FIBROBLASTS ; COLLAGEN ; POLARIZATION ; MIGRATION ; MECHANOTRANSDUCTION |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000423355300022 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/285568]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_加速器中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Chen, K,Qin, YX,Zhao, XY,et al. Mono-fullerenols modulating cell stiffness by perturbing actin bundling[J]. NANOSCALE,2018,10(4):1750-1758. |
| APA | Chen, K.,Qin, YX.,Zhao, XY.,Xia, SB.,Zhang, JX.,...&秦艳霞.(2018).Mono-fullerenols modulating cell stiffness by perturbing actin bundling.NANOSCALE,10(4),1750-1758. |
| MLA | Chen, K,et al."Mono-fullerenols modulating cell stiffness by perturbing actin bundling".NANOSCALE 10.4(2018):1750-1758. |
入库方式： OAI收割
来源：高能物理研究所
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