Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer
文献类型：期刊论文
| 作者 | Feng WY(丰伟悦) ; Cai, CX; Wang, MZ; Wang, LM; Wang B(汪冰); Ghen, CY; Wang, B; Feng, WY
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| 刊名 | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 18期号:8页码:5542-5550 |
| 关键词 | Gold Nanoparticles PEGylation Lysozyme Synchrotron Radiation Circular Dichroism |
| ISSN号 | 1533-4880 |
| DOI | 10.1166/jnn.2018.15416 |
| 文献子类 | Article |
| 英文摘要 | Characterization of bio-nano interface is crucial for developing safer and more efficient nanoparticles in nanomedical application. PEGylation is commonly used in nanocarrier for drug delivery, as it confers nanoparticles good stability, stealth effect and better targeting specificity compared with those without PEGylation. However, the protein binding state on PEGylated AuNP is still limited known. In present work, we prepared 13 nm AuNPs and then PEGylated them with thiol PEG methoxy. Lysozyme is selected as a model protein and to investigate the interactions on protein-PEGylated/AuNP interface. The thermal unfolding processes of lysozyme in absence and presence of PEGylated AuNP were measured by synchrotron radiation based circular dichroism (SRCD), dynamic light scattering (DLS) and infrared spectroscopy (IR). The results suggest that in terms of secondary structural changes, alpha helix content is decreased, while beta sheet content is increased, and thus the adsorbed lysozyme may be present in PEG layer. |
| 电子版国际标准刊号 | 1533-4899 |
| WOS关键词 | TRANSFORM-INFRARED-SPECTROSCOPY ; CIRCULAR-DICHROISM SPECTROSCOPY ; SECONDARY STRUCTURE ANALYSES ; PROTEIN ADSORPTION ; CONFORMATIONAL-CHANGES ; THERMODYNAMICS ; SURFACES |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000426059800042 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/285686]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 通讯作者 | Feng WY(丰伟悦) |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Feng WY,Cai, CX,Wang, MZ,et al. Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2018,18(8):5542-5550. |
| APA | 丰伟悦.,Cai, CX.,Wang, MZ.,Wang, LM.,汪冰.,...&Feng, WY.(2018).Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,18(8),5542-5550. |
| MLA | 丰伟悦,et al."Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 18.8(2018):5542-5550. |
入库方式： OAI收割
来源：高能物理研究所
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