Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers
文献类型：期刊论文
| 作者 | Song, CY; Fan, C; Jiang, HFD; Han YB(韩雨孛) ; Kim, C; Kim, Y; Liu, HJ; Sun, ZB; Fan, JD; Li, HY
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| 刊名 | ACS NANO
 |
| 出版日期 | 2018 |
| 卷号 | 12期号:8页码:7509-7518 |
| 关键词 | DNA origami X-ray free-electron laser single particle imaging coherent diffraction imaging X-ray diffraction |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/acsnano.8b01838 |
| 文献子类 | Article |
| 英文摘要 | It has been proposed that the radiation damage to biological particles and soft condensed matter can be overcome by ultrafast and ultraintense X-ray free-electron lasers (FELs) with short pulse durations. The successful demonstration of the "diffraction-before-destruction" concept has made single-shot diffraction imaging a promising tool to achieve high resolutions under the native states of samples. However, the resolution is still limited because of the low signal-to-noise ratio, especially for biological specimens such as cells, viruses, and macromolecular partides. Here, we present a demonstration single-shot diffraction imaging experiment of DNA-based structures at SPring-8 Angstrom Compact Free Electron Laser (SACLA), Japan. Through quantitative analysis of the reconstructed images, the scattering abilities of gold and DNA were demonstrated. Suggestions for extracting valid DNA signals from noisy diffraction patterns were also explained and outlined. To sketch out the necessary experimental conditions for the 3D imaging of DNA origami or DNA macromolecular particles, we carried out numerical simulations with practical detector noise and experimental geometry using the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory, USA. The simulated results demonstrate that it is possible to capture images of DNA-based structures at high resolutions with the technique development of current and next-generation X-ray FEL facilities. |
| 电子版国际标准刊号 | 1936-086X |
| WOS关键词 | COHERENT-LIGHT SOURCE ; PLASMONIC NANOSTRUCTURES ; ORIGAMI ; CRYSTALLOGRAPHY ; PULSES ; RECONSTRUCTION ; NANOPARTICLES ; ORIENTATION ; INSTRUMENT ; RESOLUTION |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000443525600007 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/286286]  |
| 专题 | 高能物理研究所_加速器中心 高能物理研究所_实验物理中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Song, CY,Fan, C,Jiang, HFD,et al. Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers[J]. ACS NANO,2018,12(8):7509-7518. |
| APA | Song, CY.,Fan, C.,Jiang, HFD.,韩雨孛.,Kim, C.,...&Ishikawa, T.(2018).Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers.ACS NANO,12(8),7509-7518. |
| MLA | Song, CY,et al."Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers".ACS NANO 12.8(2018):7509-7518. |
入库方式： OAI收割
来源：高能物理研究所
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