Quantitative Analysis of Multiple Proteins of Different Invasive Tumor Cell Lines at the Same Single-Cell Level
文献类型：期刊论文
| 作者 | Gao, XY ; Xing, GM; Yuan, Q; Shu, QM; Liu, R; Zhang, XC; Gao XY(高学云); Xing GM(邢更妹); Yuan Q(袁卿); Liu R(刘茹)
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| 刊名 | SMALL
 |
| 出版日期 | 2018 |
| 卷号 | 14期号:17页码:1703684 |
| 关键词 | integrin alpha(V)beta(3) invasion tumors metal clusters MT1-MMP single cells |
| ISSN号 | 1613-6810 |
| DOI | 10.1002/smll.201703684 |
| 文献子类 | Article |
| 英文摘要 | Tumor cell invasion is pivotal to the development, metastasis, and prognosis of tumors. It is reported that the invasive ability of tumor cells is mainly dependent on the expression levels of membrane type-1 matrix metalloproteinase (MT1-MMP) and integrin alpha(V)beta(3) proteins on cell membranes. To precisely distinguish between tumor cells with different invasive abilities, it is important to establish a highly sensitive and precise quantification method to differentiate the expression levels of MT1-MMP and integrin alpha(V)beta(3) in the same single tumor cell at the same time. Herein, two functional peptides to construct red-emissive Au-26 clusters and green-emissive Ag-12 clusters are reported. Moreover, the Au-26 clusters and Ag-12 clusters have the ability to specifically target MT1-MMP and integrin alpha(V)beta(3), respectively, in the same single cell at the same time. By utilizing the fluorescent properties and metallic compositions of metal clusters, the MT1-MMP and integrin alpha(V)beta(3) levels of the more invasive SiHa cells or the less invasive HeLa cells are simultaneously and quantitatively differentiated via laser ablation inductively coupled plasma mass spectrometry. This method of quantitatively detecting multiple invasive proteins on the same cell is of great value for accurately diagnosing aggressive tumors and monitoring the invasiveness of these tumors. |
| 电子版国际标准刊号 | 1613-6829 |
| WOS关键词 | EPITHELIAL-MESENCHYMAL TRANSITION ; TYPE-1 MATRIX-METALLOPROTEINASE ; EXTRACELLULAR-MATRIX ; THERAPEUTIC TARGETS ; MASS-SPECTROMETRY ; CANCER-CELLS ; E-CADHERIN ; INTEGRIN ; METASTASIS ; ADHESION |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000430922100011 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/285857]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_粒子天体物理中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Gao, XY,Xing, GM,Yuan, Q,et al. Quantitative Analysis of Multiple Proteins of Different Invasive Tumor Cell Lines at the Same Single-Cell Level[J]. SMALL,2018,14(17):1703684. |
| APA | Gao, XY.,Xing, GM.,Yuan, Q.,Shu, QM.,Liu, R.,...&张相春.(2018).Quantitative Analysis of Multiple Proteins of Different Invasive Tumor Cell Lines at the Same Single-Cell Level.SMALL,14(17),1703684. |
| MLA | Gao, XY,et al."Quantitative Analysis of Multiple Proteins of Different Invasive Tumor Cell Lines at the Same Single-Cell Level".SMALL 14.17(2018):1703684. |
入库方式： OAI收割
来源：高能物理研究所
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