Assessment of the MT1-MMP expression level of different cell lines by the naked eye
文献类型：期刊论文
| 作者 | Gao, XY ; Zhang XC(张祥春); Yuan Q(袁卿); Gao XY(高学云); Zhagn, XC; Yuan, Q
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| 刊名 | SCIENCE CHINA-LIFE SCIENCES
 |
| 出版日期 | 2018 |
| 卷号 | 61期号:4页码:492-500 |
| 关键词 | Au clusters MT1-MMP catalyze tumor cells |
| ISSN号 | 1674-7305 |
| DOI | 10.1007/s11427-017-9261-9 |
| 文献子类 | Article |
| 英文摘要 | Membrane type 1 matrix metalloproteinase (MT1-MMP) is expressed in most tumors and is believed to play a key role in the development, invasion and metastasis of tumors. There is an urgent need to develop a simple method to detect the MT1-MMP expression level on cells. In this current study, we demonstrated a red emission Au cluster probes with the specific targeting of MT1-MMP on human dopaminergic neuroblastoma (SH-SY5Y) cells. More importantly, utilizing the intrinsic enzyme-like activity of the Au cluster probes, the expression level of MT1-MMP on the SH-SY5Y cells could be assessed by the naked eye without cell lysis and protein extraction process. Furthermore, SH-SY5Y, human breast cancer (MCF-7), and human bronchial epithelial (16-HBE) cell lines with different MT1-MMP expression level could be distinguished using the Au cluster probes by the naked eye. Meanwhile, fluorescence intensity and Au count determined by inductively coupled plasma mass spectrometry (ICP-MS) were used to verify the feasibility of this simple analytical method. Our proposed method is rapid, convenient, and accurate and could be assessed by the naked eye. This visual assessment method of tumor-associated proteins has immense implications in sorting tumor cells. |
| 电子版国际标准刊号 | 1869-1889 |
| WOS关键词 | MATRIX METALLOPROTEINASES ; CANCER-CELLS ; MEMBRANE ; PROBE ; MICROENVIRONMENT ; CARCINOMA ; MARKING ; NUCLEI ; ENZYME |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| CSCD记录号 | CSCD:6277465 |
| WOS记录号 | WOS:000431976200014 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/285890]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_粒子天体物理中心 |
| 通讯作者 | Gao XY(高学云) |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Gao, XY,Zhang XC,Yuan Q,et al. Assessment of the MT1-MMP expression level of different cell lines by the naked eye[J]. SCIENCE CHINA-LIFE SCIENCES,2018,61(4):492-500. |
| APA | Gao, XY,张祥春,袁卿,高学云,Zhagn, XC,&Yuan, Q.(2018).Assessment of the MT1-MMP expression level of different cell lines by the naked eye.SCIENCE CHINA-LIFE SCIENCES,61(4),492-500. |
| MLA | Gao, XY,et al."Assessment of the MT1-MMP expression level of different cell lines by the naked eye".SCIENCE CHINA-LIFE SCIENCES 61.4(2018):492-500. |
入库方式： OAI收割
来源：高能物理研究所
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