Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability
文献类型：期刊论文
| 作者 | Zhao, LN ; Li, C; Zou CL(邹常玲); Zhao LN(赵丽娜); Feng, XD; Wang, XY; Han, BJ; Zou, CL; Hon, YH
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| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY LETTERS
 |
| 出版日期 | 2018 |
| 卷号 | 9期号:16页码:4638-4645 |
| ISSN号 | 1948-7185 |
| DOI | 10.1021/acs.jpclett.8b01570 |
| 文献子类 | Article |
| 英文摘要 | N-glycosylation has critical roles in regulating protein stability, but the molecular basis is poorly understood. In this study, we integrated experimental and computational techniques to investigate the mechanism by which full-length N-glycans modulate protein stability from quaternary structure perspective. We found the two inherent N-glycans of beta-glucuronidase expressed in Pichia pastoris function as "glyco-linkers" that hold spatially proximal motifs together to compact the local protein structure. We further designed and placed glyco-linkers in the unusual form of glyco-bridge and glyco-hairpin at the interfaces between domains and monomers with higher structural level, respectively, which conferred dramatically higher kinetic stability and thermodynamic stability than the inherent N-glycans. Our study not only provides unique insight into the interactions between glycans and proteins from a quaternary structure perspective but also facilitates the rational design of N-glycans as general tools that can enhance protein stability. |
| WOS关键词 | ENHANCED AROMATIC SEQUONS ; N-GLYCOSYLATION ; THERMOSTABILITY ; STABILIZATION ; CARBOHYDRATE ; SPECIFICITY ; CELLULOSE ; ENZYME |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000442446900026 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/286241]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 通讯作者 | Zhao LN(赵丽娜) |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, LN,Li, C,Zou CL,et al. Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability[J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2018,9(16):4638-4645. |
| APA | Zhao, LN.,Li, C.,邹常玲.,赵丽娜.,Feng, XD.,...&Hon, YH.(2018).Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability.JOURNAL OF PHYSICAL CHEMISTRY LETTERS,9(16),4638-4645. |
| MLA | Zhao, LN,et al."Design of Glyco-Linkers at Multiple Structural Levels to Modulate Protein Stability".JOURNAL OF PHYSICAL CHEMISTRY LETTERS 9.16(2018):4638-4645. |
入库方式： OAI收割
来源：高能物理研究所
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