Resonance light scattering aptasensor for urinary 8-hydroxy-2 '-deoxyguanosine based on magnetic nanoparticles: a preliminary study of oxidative stress association with air pollution
文献类型：期刊论文
| 作者 | Chen CY(陈春英); Li, CZ; Chen, CY; Wang, YP; Hou, YN; Tao, LX; Yue, QL |
| 刊名 | MICROCHIMICA ACTA
 |
| 出版日期 | 2018 |
| 卷号 | 185期号:9页码:419 |
| ISSN号 | 0026-3672 |
| 关键词 | 8-OHdG Biomarker Oxidation of DNA Aptamer Conformational change G-quadruplex Magnetic biosensor Reusability Urine samples Air quality index |
| DOI | 10.1007/s00604-018-2937-9 |
| 文献子类 | Article |
| 英文摘要 | An aptamer based method is described for the determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG) using resonance light scattering (RLS). Magnetic nanoparticles (MNPs) were employed as RLS probes. The probe DNA was placed on the surface of MNPs, which produces a rather low RLS signal. If, however, probe DNA hybridizes with the aptamer against 8-OHdG, a sandwich structure will be formed. This results in a significant enhancement of RLS intensity. The aptamer was used as the recognition element to capture 8-OHdG. 8-OHdG has a stronger affinity for the aptamer than probe DNA, and the conformation of the aptamer therefore switches from a double-stranded to a G-quadruplex structure. As a result, MNPs labeled with probe DNA are released, and RLS intensity decreases. The method allows 8-OHdG to be detected with a linear response in the 32 pM - 12.0 nM concentration range and an 11 pM limit of detection (at 3.29S (B)/m, according to the recent recommendation of IUPAC). The MNPs can be reused 5 times by applying an external magnetic field for collection. The method was successfully applied to analyze human urine samples for its content of 8-OHdG. It was also found that the levels of 8-OHdG noticeably increased with the increase of the Air Quality Index. Conceivably, the method is a viable tool to investigate the relationship between 8-OHdG levels and the effect of air pollution. |
| 电子版国际标准刊号 | 1436-5073 |
| WOS关键词 | GOLD NANOPARTICLES ; DNA-DAMAGE ; 8-HYDROXY-2-DEOXYGUANOSINE ; NANOCLUSTERS ; SPECTROSCOPY ; AGGREGATION ; BIOSENSOR ; APTAMER ; WORKERS ; CANCER |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000441932100001 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/286270]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Chen CY,Li, CZ,Chen, CY,et al. Resonance light scattering aptasensor for urinary 8-hydroxy-2 '-deoxyguanosine based on magnetic nanoparticles: a preliminary study of oxidative stress association with air pollution[J]. MICROCHIMICA ACTA,2018,185(9):419. |
| APA | 陈春英.,Li, CZ.,Chen, CY.,Wang, YP.,Hou, YN.,...&Yue, QL.(2018).Resonance light scattering aptasensor for urinary 8-hydroxy-2 '-deoxyguanosine based on magnetic nanoparticles: a preliminary study of oxidative stress association with air pollution.MICROCHIMICA ACTA,185(9),419. |
| MLA | 陈春英,et al."Resonance light scattering aptasensor for urinary 8-hydroxy-2 '-deoxyguanosine based on magnetic nanoparticles: a preliminary study of oxidative stress association with air pollution".MICROCHIMICA ACTA 185.9(2018):419. |
入库方式： OAI收割
来源：高能物理研究所
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