Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology
文献类型：期刊论文
| 作者 | Xie, GC; Gao, SJ; Shi, HG; Zhou, MX; Zhou MX(周梦雪); Tian, Y; Chen J(陈俊) ; Niu, ZW; Chen, J; Wu, M
|
| 刊名 | NANO LETTERS
 |
| 出版日期 | 2018 |
| 卷号 | 18期号:9页码:5453-5460 |
| 关键词 | Tobacco mosaic virus cell-penetrating peptide gene silence endosomal escape viral vector |
| ISSN号 | 1530-6984 |
| DOI | 10.1021/acs.nanolett.8b01805 |
| 文献子类 | Article |
| 英文摘要 | Inspired by the high gene transfer efficiency of viral vectors and to avoid side effects, we present here a 1D rod-like gene-silencing vector based on a plant virus. By decorating the transacting activator of transduction (TAT) peptide on the exterior surface, the TAT-modified tobacco mosaic virus (TMV) achieves a tunable isoelectric point (from similar to 3.5 to similar to 9.6) depending on the TAT dose. In addition to enhanced cell internalization, this plant virus based vector (TMV-TAT) acquired endo/lysosomal escape capacity without inducing lysosomal damage, resulting in both high efficiency and low cytotoxicity. By loading silencer green fluorescent protein (GFP) siRNA onto the TMV-TAT vector (siRNA@TMV-TAT) and interfering with GFP-expressing mouse epidermal stem cells (ESCs/GFP) in vitro, the proportion of GFP-positive cells could be knocked down to levels even lower than 15% at a concentration of similar to 100% cell viability. Moreover, by interfering with GFP-expressing highly metastatic hepatocellular carcinoma (MHCC97-H/GFP) tumors in vivo, treatment with siRNA@TMV-TAT complexes for 10 days achieved a GFP-negative rate as high as 80.8%. This work combines the high efficiency of viral vectors and the safety of nonviral vectors and may provide a promising strategy for gene-silencing technology. |
| 电子版国际标准刊号 | 1530-6992 |
| WOS关键词 | TOBACCO-MOSAIC-VIRUS ; MULTIWALL CARBON NANOTUBES ; RNA INTERFERENCE ; DRUG-DELIVERY ; NANOPARTICLES ; VECTOR ; FLUORESCENCE ; THERAPY ; PROTEINS ; CARRIERS |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000444793500016 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/286333]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Xie, GC,Gao, SJ,Shi, HG,et al. Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology[J]. NANO LETTERS,2018,18(9):5453-5460. |
| APA | Xie, GC.,Gao, SJ.,Shi, HG.,Zhou, MX.,周梦雪.,...&Zhu, M.(2018).Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology.NANO LETTERS,18(9),5453-5460. |
| MLA | Xie, GC,et al."Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology".NANO LETTERS 18.9(2018):5453-5460. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。