Protein Conjugation with Gold Nanoparticles: Spectroscopic and Thermodynamic Analysis on the Conformational and Activity of Serum Albumin
文献类型：期刊论文
| 作者 | Xu, C ; Zhao, XC; Wang, L; Luo, YJ; Zhang, XC; Wang, YL; Lan, J; Wang YL(王亚玲)
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| 刊名 | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
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| 出版日期 | 2018 |
| 卷号 | 18期号:11页码:7818-7823 |
| 关键词 | Gold Nanoparticles Human Serum Albumin Spectroscopy Conformation Activity |
| ISSN号 | 1533-4880 |
| DOI | 10.1166/jnn.2018.15215 |
| 文献子类 | Article |
| 英文摘要 | Gold nanoparticles (GNPs) have large scale application in many important areas with potential exposure to the environment or organisms during their life cycle. This article identifies the binding mechanism of cetyltrimethyl ammonium bromide (CTAB) coated GNPs to human serum albumin (HSA) utilizing fluorescence, UV-vis, circular dichroism (CD) spectroscopy and isothermal titration calorimetry (ITC) techniques. The fluorescence research presented the intrinsic fluorescence of HSA was dampened due to the adding of GNPs under a static dampening process. The thermodynamic study further showed the binding interaction was controlled by van der Waals forces or hydrogen bonds (Delta H and Delta S are -3.06x10(7) J.mol(-1) and -1.03x10(5) J.mol(-1).K-1, respectively) for the most part. On the other hand, UV-vis, CD spectroscopy and esterase-like activity studies confirmed the conformational changes and activity reduction of HSA. This research presented reasonable models, which facilitate our understanding of the transporting, circulation and toxic effects of GNPs after spreading into human blood. |
| 电子版国际标准刊号 | 1533-4899 |
| WOS关键词 | CARBON NANOTUBES ; DEPENDENCE ; BINDING ; CORONA ; SIZE |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000443946600072 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/286348]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_加速器中心 |
| 通讯作者 | Wang YL(王亚玲) |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Xu, C,Zhao, XC,Wang, L,et al. Protein Conjugation with Gold Nanoparticles: Spectroscopic and Thermodynamic Analysis on the Conformational and Activity of Serum Albumin[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2018,18(11):7818-7823. |
| APA | Xu, C.,Zhao, XC.,Wang, L.,Luo, YJ.,Zhang, XC.,...&王亚玲.(2018).Protein Conjugation with Gold Nanoparticles: Spectroscopic and Thermodynamic Analysis on the Conformational and Activity of Serum Albumin.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,18(11),7818-7823. |
| MLA | Xu, C,et al."Protein Conjugation with Gold Nanoparticles: Spectroscopic and Thermodynamic Analysis on the Conformational and Activity of Serum Albumin".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 18.11(2018):7818-7823. |
入库方式： OAI收割
来源：高能物理研究所
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