Selenoprotein P as the major transporter for mercury in serum from methylmercury-poisoned rats
文献类型：期刊论文
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| 作者 | 刘洋(多); Zhang W(张伟) ; Zhao JT(赵甲亭); Lin XY(林晓影); Cui LW(崔丽巍); Gao YX(高愈希); Li B(李柏); Li YF(李玉锋); Liu, Yang; Zhang, Wei
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| 刊名 | JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
 ; JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
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| 出版日期 | 2018 ; 2018 |
| 卷号 | 50页码:589-595 |
| 关键词 | Methylmercury Methylmercury Selenium Selenoprotein P Isotope dilution Selenium Selenoprotein P Isotope dilution |
| ISSN号 | 0946-672X ; 0946-672X |
| DOI | 10.1016/j.jtemb.2018.04.013 ; 10.1016/j.jtemb.2018.04.013 |
| 文献子类 | Article; Proceedings Paper ; Article; Proceedings Paper |
| 英文摘要 | Selenium (Se) has been found to promote weight gain, decrease hepatic damage, but redistribute mercury (Hg) in brains and livers in methylmercury (MeHg)-poisoned rats. The aims of the present work were to examine the effects of Se on the levels of Hg in serum and the role of serum selenoproteins in binding with Hg in MeHg-poisoned rats. The concentration of Se, Hg and MeHg were studied using ICP-MS and CVAFS. The Hg- and Se binding selenoproteins were separated and quantified using affinity chromatography with post-column isotope dilution analysis using both enriched Se-78 and Hg-199. It was found that Se treatment reduced Hg levels in serum in MeHg-poisoned rats. Among the three separated selenoproteins, the amounts of SelP-bound Hg and Se increased to 73% and 93.6%, from 64.4% and 89.3% of the total Hg and Se, respectively after Se treatment, suggesting that SelP acts as a major transporter for Hg and pool for Se in serum. Over 90% of the total Hg was MeHg in serum, and the molar ratios of MeHg to Se as 1:4 and 1:9 in the formed MeHg-Se-SelP complex in the control and the Se treatment group, respectively. The elevated Se level binding with SelP facilitated the Hg extraction from tissues and organs, as well as its redistribution in brains and livers through blood circulation in the MeHg-poisoned rats. Together, our findings provide direct evidence that serum SelP is the major Hg transporter in MeHg-poisoned rats.; Selenium (Se) has been found to promote weight gain, decrease hepatic damage, but redistribute mercury (Hg) in brains and livers in methylmercury (MeHg)-poisoned rats. The aims of the present work were to examine the effects of Se on the levels of Hg in serum and the role of serum selenoproteins in binding with Hg in MeHg-poisoned rats. The concentration of Se, Hg and MeHg were studied using ICP-MS and CVAFS. The Hg- and Se binding selenoproteins were separated and quantified using affinity chromatography with post-column isotope dilution analysis using both enriched Se-78 and Hg-199. It was found that Se treatment reduced Hg levels in serum in MeHg-poisoned rats. Among the three separated selenoproteins, the amounts of SelP-bound Hg and Se increased to 73% and 93.6%, from 64.4% and 89.3% of the total Hg and Se, respectively after Se treatment, suggesting that SelP acts as a major transporter for Hg and pool for Se in serum. Over 90% of the total Hg was MeHg in serum, and the molar ratios of MeHg to Se as 1:4 and 1:9 in the formed MeHg-Se-SelP complex in the control and the Se treatment group, respectively. The elevated Se level binding with SelP facilitated the Hg extraction from tissues and organs, as well as its redistribution in brains and livers through blood circulation in the MeHg-poisoned rats. Together, our findings provide direct evidence that serum SelP is the major Hg transporter in MeHg-poisoned rats. |
| 会议地点 | St Petersburg, RUSSIA ; St Petersburg, RUSSIA |
| WOS关键词 | SELENIUM ; SELENIUM |
| WOS研究方向 | Astronomy & Astrophysics ; Astronomy & Astrophysics |
| 语种 | 英语 ; 英语 |
| WOS记录号 | WOS:000448633900079 ; WOS:000448633900079 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/286698]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_实验物理中心 高能物理研究所_加速器中心 高能物理研究所_东莞分部 |
| 通讯作者 | Li YF(李玉锋) |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Liu Y,Zhang W,Zhao JT,et al. Selenoprotein P as the major transporter for mercury in serum from methylmercury-poisoned rats, Selenoprotein P as the major transporter for mercury in serum from methylmercury-poisoned rats[J]. JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY, JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY,2018, 2018,50, 50:589-595, 589-595. |
| APA | 刘洋.,张伟.,赵甲亭.,林晓影.,崔丽巍.,...&Li, Yu-Feng.(2018).Selenoprotein P as the major transporter for mercury in serum from methylmercury-poisoned rats.JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY,50,589-595. |
| MLA | 刘洋,et al."Selenoprotein P as the major transporter for mercury in serum from methylmercury-poisoned rats".JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY 50(2018):589-595. |
入库方式： OAI收割
来源：高能物理研究所
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