Structural Basis for Recognition of a Unique Epitope by a Human Anti-tau Antibody
文献类型：期刊论文
| 作者 | Zhu, X.; Wilson, I. A.; Koudstaal, W.; Apetri, A.; Goudsmit, J.; Stoop, E. J. M.; Inganas, H.; Siregar, B.; Hoozemans, J. J.; Keogh, E. |
| 刊名 | STRUCTURE
 |
| 出版日期 | 2018 |
| 卷号 | 26期号:12页码:1626-1634 |
| ISSN号 | 0969-2126 |
| DOI | 10.1016/j.str.2018.08.012 |
| 文献子类 | Article |
| 英文摘要 | Aggregation of the hyperphosphorylated protein tau into neurofibrillary tangles and neuropil threads is a hallmark of Alzheimer disease (AD). Identification and characterization of the epitopes recognized by anti-tau antibodies might shed light on the molecular mechanisms of AD pathogenesis. Here we report on the biochemical and structural characterization of a tau-specific monoclonal antibody CBTAU-24.1, which was isolated from the human memory B cell repertoire. Immunohistochemical staining with CBTAU-24.1 specifically detects pathological tau structures in AD brain samples. The crystal structure of CBTAU-24.1 Fab with a phosphorylated tau peptide revealed recognition of a unique epitope (Ser235-Leu243) in the tau proline-rich domain. Interestingly, the antibody can bind tau regardless of phosphorylation state of its epitope region and also recognizes both monomeric and paired helical filament tau irrespective of phosphorylation status. This human anti-tau antibody and its unique epitope may aid in development of diagnostics and/or therapeutic AD strategies. |
| 电子版国际标准刊号 | 1878-4186 |
| WOS关键词 | PAIRED HELICAL FILAMENTS ; PHOSPHORYLATED TAU ; ALZHEIMERS-DISEASE ; PROTEIN ; PATHOLOGY ; STATE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000454803300009 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/286861]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, X.,Wilson, I. A.,Koudstaal, W.,et al. Structural Basis for Recognition of a Unique Epitope by a Human Anti-tau Antibody[J]. STRUCTURE,2018,26(12):1626-1634. |
| APA | Zhu, X..,Wilson, I. A..,Koudstaal, W..,Apetri, A..,Goudsmit, J..,...&张衡.(2018).Structural Basis for Recognition of a Unique Epitope by a Human Anti-tau Antibody.STRUCTURE,26(12),1626-1634. |
| MLA | Zhu, X.,et al."Structural Basis for Recognition of a Unique Epitope by a Human Anti-tau Antibody".STRUCTURE 26.12(2018):1626-1634. |
入库方式： OAI收割
来源：高能物理研究所
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