The Precise Diagnosis of Cancer Invasion/Metastasis via 2D Laser Ablation Mass Mapping of Metalloproteinase in Primary Cancer Tissue
文献类型：期刊论文
| 作者 | Zhang XC(张祥春); Gao, Xueyun ; Zhang, Xiangchun, Liu, Ru, Yuan, Qing, Gao, Fuping, Li, Jiaojiao, Zhang, Ya, Zhao, Yuliang, Chai, Zhifang, Gao, Liang; Gao, Xueyun; Gao, Liang; Chai, Zhifang; Zhao, Yuliang; Zhang, Ya; Li, Jiaojiao; Gao, Fuping
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| 刊名 | ACS NANO
 |
| 出版日期 | 2018 |
| 卷号 | 12期号:11页码:11139-11151 |
| 关键词 | primary tumor invasion/metastasis Au clusters MT1-MMP 2D-LA-Mass Mapping lung carcinoma renal carcinoma |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/acsnano.8b05584 |
| 文献子类 | Article |
| 英文摘要 | Cancer invasion and metastasis, remain the major causes of over 90% of patient deaths. Molecular imaging methods such as computed tomography (CT)/magnetic resonance imaging (MRI) can precisely assess primary regional lymph node invasion and distant organ metastasis via body scanning; however, such diagnostic methods are often utilized too late for cancer therapy. To date, pathologic methods mainly provide information on differentiation/proliferation and potential drug therapy biomarkers of primary tumors rather than precisely reveal tumor regional invasion and distant metastasis in the body. We hypothesized that quantification of membrane type-1 matrix metalloproteinase (MT1-MMP) levels in primary tumor tissue will provide a precise assessment of tumor regional lymph node invasion and remote organ metastasis. In this work, we developed peptide-coated Au clusters with intrinsic red fluorescence and a specific mass signal. When these clusters labeled MT1-MMP in tumor tissue sections derived from the xenograft lung carcinoma model, human lung carcinoma and human renal carcinoma, we could directly observe MT1-MMP via optical fluorescence microscopy and quantitatively detect the MT1-MMP expression level via laser ablation inductively coupled plasma mass spectrometry 2D mapping (2D-LA-Mass Mapping). By observing and quantifying the MT1-MMP expression level in primary human lung carcinoma and human renal carcinoma tissue sections, we precisely assessed the risk of primary tumor invasion/metastasis. Importantly, the accuracy of this pathologic method was verified by CT/MRI molecular imaging of cancer patients and traditional hematoxylin and eosin (H&E) staining/immunohistochemistry (IHC)/immunofluorescence (IF) pathologic studies of primary tumor tissues. |
| 电子版国际标准刊号 | 1936-086X |
| WOS关键词 | MATRIX METALLOPROTEINASES ; TUMOR INVASION ; EXTRACELLULAR-MATRIX ; LUNG-CANCER ; METASTASIS ; MT1-MMP ; EXPRESSION ; NODE ; NANOCLUSTERS ; INSIGHTS |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000451789200053 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/286874]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhang XC,Gao, Xueyun,Zhang, Xiangchun, Liu, Ru, Yuan, Qing, Gao, Fuping, Li, Jiaojiao, Zhang, Ya, Zhao, Yuliang, Chai, Zhifang, Gao, Liang,et al. The Precise Diagnosis of Cancer Invasion/Metastasis via 2D Laser Ablation Mass Mapping of Metalloproteinase in Primary Cancer Tissue[J]. ACS NANO,2018,12(11):11139-11151. |
| APA | 张祥春.,Gao, Xueyun.,Zhang, Xiangchun, Liu, Ru, Yuan, Qing, Gao, Fuping, Li, Jiaojiao, Zhang, Ya, Zhao, Yuliang, Chai, Zhifang, Gao, Liang.,Gao, Xueyun.,Gao, Liang.,...&刘茹.(2018).The Precise Diagnosis of Cancer Invasion/Metastasis via 2D Laser Ablation Mass Mapping of Metalloproteinase in Primary Cancer Tissue.ACS NANO,12(11),11139-11151. |
| MLA | 张祥春,et al."The Precise Diagnosis of Cancer Invasion/Metastasis via 2D Laser Ablation Mass Mapping of Metalloproteinase in Primary Cancer Tissue".ACS NANO 12.11(2018):11139-11151. |
入库方式： OAI收割
来源：高能物理研究所
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