Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach
文献类型：期刊论文
| 作者 | Chen CF(陈超飞); Xie JN(谢佳妮); Li T(李婷); Li YR(李玉茹); Liu L(刘蕾); Liu, QS; Chen, CF; Xie, JI; Li, T; Li, YR |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016 |
| 卷号 | 88期号:2页码:1073-1077 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.5b04102 |
| 文献子类 | Article |
| 英文摘要 | We have developed a nanopore sensing approach for the selective detection of 8-oxo-2'-deoxyguanosine (8-oxoG) in single-stranded DNA. First, 1,12-dodecanediamine is coupled with 8-oxoG-containing DNA molecules in high yield which leaves a free amine group for subsequent attaching of an adamantane moiety. After incubation with cucurbit[7]uril, the host-guest complex-modified DNA hybrid is translocated through an alpha-hemolysin nanopore. Highly characteristic events can be recorded and used to quantify the 8-oxoG-DNA content in a DNA mixture. Compared with the existing methods, this study provides a reliable, quick, and low-cost approach for the detection of 8-oxoG site in single-stranded DNA at the single-molecule level, particularly suitable for high-throughput screening of a massive number of samples. |
| 电子版国际标准刊号 | 1520-6882 |
| 语种 | 英语 |
| WOS记录号 | WOS:000368562300002 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/287071]  |
| 专题 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Chen CF,Xie JN,Li T,et al. Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach[J]. ANALYTICAL CHEMISTRY,2016,88(2):1073-1077. |
| APA | 陈超飞.,谢佳妮.,李婷.,李玉茹.,刘蕾.,...&Zhang, SW.(2016).Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach.ANALYTICAL CHEMISTRY,88(2),1073-1077. |
| MLA | 陈超飞,et al."Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach".ANALYTICAL CHEMISTRY 88.2(2016):1073-1077. |
入库方式： OAI收割
来源：高能物理研究所
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