Genetic diversity of populus cathayana Rehd populations in southwestern China revealed by ISSR markers
文献类型:期刊论文
作者 | Lu, ZX ; Wang, YH ; Peng, YH ; Korpelainen, H ; Li, CY |
刊名 | PLANT SCIENCE
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出版日期 | 2006 |
卷号 | 170期号:2页码:407_412 |
关键词 | Qinghai-Tibetan plateau Populus cathayana Rehd genetic variation ISSR |
ISSN号 | 0168-9452 |
通讯作者 | Li, CY, Chinese Acad Sci, Chengdu Inst Biol, POB 416, Chengdu 610041, Peoples R China. |
中文摘要 | As a native species to China, Populus cathayana Rehd is well-adapted to the wet and cold environments where it occurs. It is considered to be an important reforestation species in western China. In the present study, we surveyed the level of genetic variation and the pattern of genetic structure in seven natural populations of R cathayana, originating from the southeastern Qinghai-Tibetan Plateau of China, by using ISSR (inter-simple sequence repeats) markers. Based on eight primers, 158 clear and reproducible DNA fragments were generated, of which 156 (98.7%) were polymorphic. The average value of Nei's gene diversity (h) equaled 0.331. The coefficient of genetic differentiation (G(ST)) equaled 0.477, which means that 47.7% of the total molecular variance existed among populations. Such a high level of divergence present among populations may be caused by the complex topography and variable climatic conditions present in the southeastern Qinghai-Tibetan Plateau which effectively restrict gene flow. Moreover, there is a lack of significant association between genetic and geographical distances (r = 0.3122, P > 0.05) in the populations of R cathayana. The application of a novel method, which combines geographical coordinates and genetic differentiation to detect barriers for gene flow, allowed us to identify two zones of lowered gene flow. The ISSR method used in the present genetic fingerprinting-based study proved to be an effective high-resolution tool for identifying patterns of genetic variation present in forest trees. In the case of P. cathayana, such information is greatly needed for planning conservation genetic actions to ensure the maintenance of appropriate levels of genetic variation. (c) 2005 Elsevier Ireland Ltd. All rights reserved. |
学科主题 | Biochemistry & Molecular Biology; Plant Sciences |
收录类别 | SCI |
语种 | 英语 |
公开日期 | 2011-07-08 |
源URL | [http://210.75.237.14/handle/351003/21890] ![]() |
专题 | 成都生物研究所_生态研究 |
推荐引用方式 GB/T 7714 | Lu, ZX,Wang, YH,Peng, YH,et al. Genetic diversity of populus cathayana Rehd populations in southwestern China revealed by ISSR markers[J]. PLANT SCIENCE,2006,170(2):407_412. |
APA | Lu, ZX,Wang, YH,Peng, YH,Korpelainen, H,&Li, CY.(2006).Genetic diversity of populus cathayana Rehd populations in southwestern China revealed by ISSR markers.PLANT SCIENCE,170(2),407_412. |
MLA | Lu, ZX,et al."Genetic diversity of populus cathayana Rehd populations in southwestern China revealed by ISSR markers".PLANT SCIENCE 170.2(2006):407_412. |
入库方式: OAI收割
来源:成都生物研究所
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