Molecular and cellular mechanisms for zoledronic acid-loaded magnesium-strontium alloys to inhibit giant cell tumors of bone
文献类型：期刊论文
| 作者 | Li, M; Wang, WD; Zhu, Y3; Lu, Y4; Wan, P5; Yang, K1; Zhang, Y; Mao, CB |
| 刊名 | ACTA BIOMATERIALIA
 |
| 出版日期 | 2018-09-01 |
| 卷号 | 77页码:365-379 |
| ISSN号 | 1742-7061 |
| 关键词 | Magnesium-strontium alloys CaP coating Zoledronic acid Giant cell tumors of bone Mechanisms |
| DOI | 10.1016/j.actbio.2018.07.028 |
| 英文摘要 | Giant Cell Tumors of Bone (GCTB) are benign but aggressive and metastatic tumors. Surgical removal cannot eradicate GCTB due to the subsequent recurrence and osteolysis. Here we developed Zoledronic acid (ZA)-loaded magnesium-strontium (Mg-Sr) alloys that can inhibit GCTB and studied the molecular and cellular mechanisms of such inhibition. We first formed a calcium phosphate (CaP) coating on the Mg-1.5 wt%Sr implants by coprecipitation and then loaded ZA on the CaP coating. We examined the response of GCTB cells to the ZA-loaded alloys. At the cellular level, the alloys not only induced apoptosis and oxidative stress of GCTB cells, and suppressed their resultant pre-osteoclast recruitment, but also inhibited their migration. At the molecular level, the alloys could significantly activate the mitochondrial pathway and inhibit the NF-KB pathway in the GCTB cells. These collectively enable the ZA-loaded alloys to suppress GCTB cell growth and osteolysis, and thus improve our understanding of the materials-induced tumor inhibition. Our study shows that ZA-loaded alloys could be a potential implant in repairing the bone defects after tumor removal in GCTB therapy. Statement of significance In clinics, giant cell tumors of bone (GCTB) are removed by surgery. However, the resultant defects in bone still contain aggressive and metastatic GCTB cells that can recruit osteoclasts to damage bone, leading to new GCTB tumor growth and bone damage after tumor surgery. Hence, it is of high demand in developing a material that can not only fill the bone defects as an implant but also inhibit GCTB in the defect area as a therapeutic agent. More importantly, the molecular and cellular mechanism by which such a material inhibits GCTB growth has never been explored. To solve these two problems, we prepared a new biomaterial, the Mg-Sr alloys that were first coated with calcium phosphate and then loaded with a tumor-inhibiting molecule (Zoledronic acid, ZA). Then, by using a variety of molecular and cellular biological assays, we studied how the ZA-loaded alloys induced the death of GCTB cells (derived from patients) and inhibited their growth at the molecular and cellular level. At the cellular level, our results showed that ZA-loaded Mg-Sr alloys not only induced apoptosis and oxidative stress of GCTB cells, and suppressed their induced pre-osteoclast recruitment, but also inhibited their migration. At the molecular level, our data showed that ZA released from the ZA-loaded Mg-Sr alloys could significantly activate the mitochondrial pathway and inhibit the NF-kappa B pathway in the GCTB cells. Both mechanisms collectively induced GCTB cell death and inhibited GCTB cell growth. This work showed how a biomaterial inhibit tumor growth at the molecular and cellular level, increasing our understanding in the fundamental principle of materials-induced cancer therapy. This work will be interesting to readers in the fields of metallic materials, inorganic materials, biomaterials and cancer therapy. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
| 学科主题 | Engineering, Biomedical ; Materials Science, bioMaterials |
| 语种 | 英语 |
| WOS记录号 | WOS:000443668900032 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/79981]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 作者单位 | 1.Guangdong Acad Med Sci, Guangdong Gen Hosp, Dept Orthoped, Guangzhou 510080, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Univ Oklahoma, Inst Biomed Engn Sci & Technol, Stephenson Life Sci Res Ctr, Dept Chem & Biochem, Norman, OK 73019 USA 5.Guangzhou Mil Command, Guangzhou Gen Hosp, Guangdong Key Lab Orthopaed Technol & Implant Mat, Dept Orthoped, Guangzhou 510010, Guangdong, Peoples R China 6.Dongguan Univ Technol, Sch Mech Engn, Dongguan 523808, Peoples R China 7.Zhejiang Univ, Sch Mat Sci & Engn, Hangzhou 310027, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, M,Wang, WD,Zhu, Y,et al. Molecular and cellular mechanisms for zoledronic acid-loaded magnesium-strontium alloys to inhibit giant cell tumors of bone[J]. ACTA BIOMATERIALIA,2018,77:365-379. |
| APA | Li, M.,Wang, WD.,Zhu, Y.,Lu, Y.,Wan, P.,...&Mao, CB.(2018).Molecular and cellular mechanisms for zoledronic acid-loaded magnesium-strontium alloys to inhibit giant cell tumors of bone.ACTA BIOMATERIALIA,77,365-379. |
| MLA | Li, M,et al."Molecular and cellular mechanisms for zoledronic acid-loaded magnesium-strontium alloys to inhibit giant cell tumors of bone".ACTA BIOMATERIALIA 77(2018):365-379. |
入库方式： OAI收割
来源：金属研究所
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