2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol
文献类型：期刊论文
| 作者 | Wu, LX; Lu, XB; Dhanjai; Wu, ZS; Dong, YF; Wang, XH; Zheng, SH; Chen, JP; Lu, XB (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China. |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 107页码:69-75 |
| 关键词 | 2-dimensional Titanium Carbide Water-soluble Graphene Ti3c2 Mxene Functionalized Graphene Direct Electrochemistry Aqueous-media Ion Batteries Adsorption Intercalation Exfoliation |
| ISSN号 | 0956-5663 |
| 英文摘要 | MXene-Ti3C2, as a new class of two-dimensional (2D) transition metal carbides (or nitrides), has been synthesized by exfoliating pristine Ti3AlC2 phases with hydrofluoric acid. The SEM and XRD images show that the resultant MXene possesses a graphene-like 2D nanostructure. and the surface of MXene has been partially terminated with-OH, thus providing a favorable microenvironment for enzyme immobilization and retaining their bioactivity and stability. Considering the unique metallic conductivity, biocompatibility and good dispersion in aqueous phase, the as-prepared MXene was explored as a new matrix to immobilize tyrosinase (a model enzyme) for fabricating a mediator-free biosensor for ultrasensidve and rapid detection of phenol. The varying electrochemical measurements were used to investigate the electrochemical performance of MXene-based tyrosinase biosensors. The results revealed that the direct electron transfer between tyrosinase and electrode could be easily achieved via a surface-controlled electrochemical process. The fabricated MXene-based tyrosinase biosensors exhibited good analytical performance over a wide linear range from 0.05 to 15.5 mu mol L-1, with a low detection limit of 12 nmol L-1 and a sensitivity of 414.4 mA M-1. The proposed biosensing approach also demonstrated good repeatability, reproducibility, long-term stability and high recovery for phenol detection in real water samples. With those excellent performances, MXene with graphene-like structure is proved to be a robust and versatile electrochemical biosensing platform for enzyme-based biosensors and biocatalysis, and has wide potential applications in biomedical detection and environmental analysis.; MXene-Ti3C2, as a new class of two-dimensional (2D) transition metal carbides (or nitrides), has been synthesized by exfoliating pristine Ti3AlC2 phases with hydrofluoric acid. The SEM and XRD images show that the resultant MXene possesses a graphene-like 2D nanostructure. and the surface of MXene has been partially terminated with-OH, thus providing a favorable microenvironment for enzyme immobilization and retaining their bioactivity and stability. Considering the unique metallic conductivity, biocompatibility and good dispersion in aqueous phase, the as-prepared MXene was explored as a new matrix to immobilize tyrosinase (a model enzyme) for fabricating a mediator-free biosensor for ultrasensidve and rapid detection of phenol. The varying electrochemical measurements were used to investigate the electrochemical performance of MXene-based tyrosinase biosensors. The results revealed that the direct electron transfer between tyrosinase and electrode could be easily achieved via a surface-controlled electrochemical process. The fabricated MXene-based tyrosinase biosensors exhibited good analytical performance over a wide linear range from 0.05 to 15.5 mu mol L-1, with a low detection limit of 12 nmol L-1 and a sensitivity of 414.4 mA M-1. The proposed biosensing approach also demonstrated good repeatability, reproducibility, long-term stability and high recovery for phenol detection in real water samples. With those excellent performances, MXene with graphene-like structure is proved to be a robust and versatile electrochemical biosensing platform for enzyme-based biosensors and biocatalysis, and has wide potential applications in biomedical detection and environmental analysis. |
| 学科主题 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| 资助机构 | National Natural Science Foundation of China [21577139, 21475130, 51572259]; Special Fund for Agro-scientific Research in the Public Interest of China [201503108]; Exploratory Research Program of Shanxi Yanchang Petroleum (Group) CO., LTD [DICP ZZBS201708]; DICP [DICP ZZBS201708] |
| 公开日期 | 2018-06-05 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/79299]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Lu, XB (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China. |
| 推荐引用方式 GB/T 7714 | Wu, LX,Lu, XB,Dhanjai,et al. 2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol[J]. BIOSENSORS & BIOELECTRONICS,2018,107:69-75. |
| APA | Wu, LX.,Lu, XB.,Dhanjai.,Wu, ZS.,Dong, YF.,...&Lu, XB .(2018).2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol.BIOSENSORS & BIOELECTRONICS,107,69-75. |
| MLA | Wu, LX,et al."2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol".BIOSENSORS & BIOELECTRONICS 107(2018):69-75. |
入库方式： OAI收割
来源：金属研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。