Enhanced production of intracellular dextran dextrinase from Gluconobacteroxydans using statistical
文献类型：期刊论文
| 作者 | Xiangzhao Mao ; Xiaotong Liang ; Shu Wang ; Wei Dong ; Yanlong Xing ; Hualei Wang ; Lizhong Guo1 ; Dongzhi Wei |
| 刊名 | African Journal of Biotechnology
 |
| 出版日期 | 2010 |
| 卷号 | 9期号:8页码:1180-1189 |
| 关键词 | Dextran dextrinase Gluconobacter oxydans medium optimization Plackett-Burmandesign central composite design |
| ISSN号 | 1684–5315 |
| 中文摘要 | Optimization of the fermentation medium for DDase production by Gluconaobacter oxydans M5 was carried out in the shake flasks using two kinds of statistical methods. Four variables, namely glucose, tryptone, yeast extract and sodium chloride, were found to influence DDase production significantly by the Plackett-Burman screening. A four-factor five-level central composite design (CCD) was chosen to explain the combined effects of the four medium constituents. The optimum medium consisted of glucose (17.670 g/L), maltobiose (30 g/L), tryptone (12.198 g/L), yeast extract (13.528 g/L), ammonium nitrate (15 g/L), copper sulfate (0.01 g/L), zinc sulfate (0.01 g/L), and sodium chloride (0.009 g/L); the initial pH 6.0 was set prior to sterilization. The DDase yield obtained from optimized medium increased by 17-fold (0.238 U/mL) or so. Under these optimal conditions, the experimental values agreed with the predicted values, indicating that the chosen method of optimization of medium composition was efficient, relatively simple, time reducing and material saving. |
| 学科主题 | 储氢及新型纳孔材料 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2011-09-09 |
| 源URL | [http://ir.qibebt.ac.cn//handle/337004/548]  |
| 专题 | 青岛生物能源与过程研究所_储氢及新型纳孔材料团队 |
| 推荐引用方式 GB/T 7714 | Xiangzhao Mao,Xiaotong Liang,Shu Wang,et al. Enhanced production of intracellular dextran dextrinase from Gluconobacteroxydans using statistical[J]. African Journal of Biotechnology,2010,9(8):1180-1189. |
| APA | Xiangzhao Mao.,Xiaotong Liang.,Shu Wang.,Wei Dong.,Yanlong Xing.,...&Dongzhi Wei.(2010).Enhanced production of intracellular dextran dextrinase from Gluconobacteroxydans using statistical.African Journal of Biotechnology,9(8),1180-1189. |
| MLA | Xiangzhao Mao,et al."Enhanced production of intracellular dextran dextrinase from Gluconobacteroxydans using statistical".African Journal of Biotechnology 9.8(2010):1180-1189. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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