De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation
文献类型：期刊论文
| 作者 | Duan, Yangkai; Zhu, Zhi; Cai, Ke; Tan, Xiaoming; Lu, Xuefeng |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2011-05-23 |
| 卷号 | 6期号:5页码:e20265 |
| 中文摘要 | Biodiesel is a renewable alternative to petroleum diesel fuel that can contribute to carbon dioxide emission reduction and energy supply. Biodiesel is composed of fatty acid alkyl esters, including fatty acid methyl esters (FAMEs) and fatty acid ethyl esters (FAEEs), and is currently produced through the transesterification reaction of methanol (or ethanol) and triacylglycerols (TAGs). TAGs are mainly obtained from oilseed plants and microalgae. A sustainable supply of TAGs is a major bottleneck for current biodiesel production. Here we report the de novo biosynthesis of FAEEs from glucose, which can be derived from lignocellulosic biomass, in genetically engineered Escherichia coli by introduction of the ethanol-producing pathway from Zymomonas mobilis, genetic manipulation to increase the pool of fatty acyl-CoA, and heterologous expression of acyl-coenzyme A: diacylglycerol acyltransferase from Acinetobacter baylyi. An optimized fed-batch microbial fermentation of the modified E. coli strain yielded a titer of 922 mg L(-1) FAEEs that consisted primarily of ethyl palmitate, -oleate, -myristate and -palmitoleate. |
| 英文摘要 | Biodiesel is a renewable alternative to petroleum diesel fuel that can contribute to carbon dioxide emission reduction and energy supply. Biodiesel is composed of fatty acid alkyl esters, including fatty acid methyl esters (FAMEs) and fatty acid ethyl esters (FAEEs), and is currently produced through the transesterification reaction of methanol (or ethanol) and triacylglycerols (TAGs). TAGs are mainly obtained from oilseed plants and microalgae. A sustainable supply of TAGs is a major bottleneck for current biodiesel production. Here we report the de novo biosynthesis of FAEEs from glucose, which can be derived from lignocellulosic biomass, in genetically engineered Escherichia coli by introduction of the ethanol-producing pathway from Zymomonas mobilis, genetic manipulation to increase the pool of fatty acyl-CoA, and heterologous expression of acyl-coenzyme A: diacylglycerol acyltransferase from Acinetobacter baylyi. An optimized fed-batch microbial fermentation of the modified E. coli strain yielded a titer of 922 mg L(-1) FAEEs that consisted primarily of ethyl palmitate, -oleate, -myristate and -palmitoleate. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | BIOMASS ; OVERPRODUCTION ; BIOFUELS ; ALKANES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291005200044 |
| 公开日期 | 2011-09-09 |
| 源URL | [http://ir.qibebt.ac.cn//handle/337004/656]  |
| 专题 | 青岛生物能源与过程研究所_微生物代谢工程团队 |
| 作者单位 | Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Yangkai,Zhu, Zhi,Cai, Ke,et al. De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation[J]. PLOS ONE,2011,6(5):e20265. |
| APA | Duan, Yangkai,Zhu, Zhi,Cai, Ke,Tan, Xiaoming,&Lu, Xuefeng.(2011).De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation.PLOS ONE,6(5),e20265. |
| MLA | Duan, Yangkai,et al."De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation".PLOS ONE 6.5(2011):e20265. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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