Succinate production from different carbon sources under anaerobic conditions by metabolic engineered Escherichia coli strains
文献类型：期刊论文
| 作者 | Wang, Jian1; Zhu, Jiangfeng2,3; Bennett, George N.4; San, Ka-Yiu5,6 |
| 刊名 | METABOLIC ENGINEERING
 |
| 出版日期 | 2011-05-01 |
| 卷号 | 13期号:3页码:328-335 |
| 关键词 | Escherichia coli Fermentation Sucrose Glucose Fructose Conjugation Succinate |
| 中文摘要 | Succinic acid has drawn much interest as a precursor of many industrially important chemicals. Using a variety of feedstocks for the bio-production of succinic acid would be economically beneficial to future industrial processes. Escherichia coli SBS550MG is able to grow on both glucose and fructose, but not on sucrose. Therefore, we derived a SBS550MG strain bearing both the pHL413 plasmid, which contains Lactococcus lactis pycA gene, and the pUR400 plasmid, which contains the scrK, Y, A, B, and R genes for sucrose uptake and catalyzation. Succinic acid production by this modified strain and the SBS550pHL413 strain was tested on fructose, sucrose, a mixture of glucose and fructose, a mixture of glucose, fructose and sucrose, and sucrose hydrolysis solution. The modified strain can produce succinic acid efficiently from all combinations of different carbon sources tested with minimal byproduct formation and with high molar succinate yields close to that of the maximum theoretic values. The molar succinic acid yield from fructose was the highest among the carbon sources tested. Using the mixture of glucose and fructose as the carbon source resulted in slightly lower yields and much higher productivity than using fructose alone. Fermenting sucrose mixed with fructose and glucose gave a 1.76-fold higher productivity than that when sucrose was used as the sole carbon source. Using sucrose pretreated with sulfuric acid as carbon source resulted in a similar succinic acid yield and productivity as that when using the mixture of sucrose, fructose, and glucose. The results of the effect of agitation rate in aerobic phase on succinate production showed that supplying large amount of oxygen in aerobic phase resulted in higher productions of formate and acetate, and therefore lower succinate yield. This study suggests that fructose, sucrose, mixture of glucose and fructose, mixture of glucose, fructose and sucrose, or sucrose hydrolysis solution could be used for the economical and efficient production of succinic acid by our metabolic engineered E. coli strain. (C) 2011 Elsevier Inc. All rights reserved. |
| 英文摘要 | Succinic acid has drawn much interest as a precursor of many industrially important chemicals. Using a variety of feedstocks for the bio-production of succinic acid would be economically beneficial to future industrial processes. Escherichia coli SBS550MG is able to grow on both glucose and fructose, but not on sucrose. Therefore, we derived a SBS550MG strain bearing both the pHL413 plasmid, which contains Lactococcus lactis pycA gene, and the pUR400 plasmid, which contains the scrK, Y, A, B, and R genes for sucrose uptake and catalyzation. Succinic acid production by this modified strain and the SBS550pHL413 strain was tested on fructose, sucrose, a mixture of glucose and fructose, a mixture of glucose, fructose and sucrose, and sucrose hydrolysis solution. The modified strain can produce succinic acid efficiently from all combinations of different carbon sources tested with minimal byproduct formation and with high molar succinate yields close to that of the maximum theoretic values. The molar succinic acid yield from fructose was the highest among the carbon sources tested. Using the mixture of glucose and fructose as the carbon source resulted in slightly lower yields and much higher productivity than using fructose alone. Fermenting sucrose mixed with fructose and glucose gave a 1.76-fold higher productivity than that when sucrose was used as the sole carbon source. Using sucrose pretreated with sulfuric acid as carbon source resulted in a similar succinic acid yield and productivity as that when using the mixture of sucrose, fructose, and glucose. The results of the effect of agitation rate in aerobic phase on succinate production showed that supplying large amount of oxygen in aerobic phase resulted in higher productions of formate and acetate, and therefore lower succinate yield. This study suggests that fructose, sucrose, mixture of glucose and fructose, mixture of glucose, fructose and sucrose, or sucrose hydrolysis solution could be used for the economical and efficient production of succinic acid by our metabolic engineered E. coli strain. (C) 2011 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | PYRUVATE-CARBOXYLASE ; ACID PRODUCTION ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; ANAEROBIOSPIRILLUM-SUCCINICIPRODUCENS ; CORYNEBACTERIUM-GLUTAMICUM ; MANNHEIMIA-SUCCINICIPRODUCENS ; ACTINOBACILLUS-SUCCINOGENES ; ENHANCED PRODUCTION ; AQUEOUS-SOLUTION ; FLUX ANALYSIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290020500008 |
| 公开日期 | 2011-09-09 |
| 源URL | [http://ir.qibebt.ac.cn//handle/337004/660]  |
| 专题 | 青岛生物能源与过程研究所_微生物代谢工程团队 |
| 作者单位 | 1.Jilin Univ, Coll Agr & Biol Engn, Changchun 130023, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 3.Chinese Acad Sci, Key Lab Biofuels, Qingdao, Peoples R China 4.Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77251 USA 5.Rice Univ, Dept Chem & Biomol Engn, Houston, TX USA 6.Rice Univ, Dept Bioengn, Houston, TX 77005 USA |
| 推荐引用方式 GB/T 7714 | Wang, Jian,Zhu, Jiangfeng,Bennett, George N.,et al. Succinate production from different carbon sources under anaerobic conditions by metabolic engineered Escherichia coli strains[J]. METABOLIC ENGINEERING,2011,13(3):328-335. |
| APA | Wang, Jian,Zhu, Jiangfeng,Bennett, George N.,&San, Ka-Yiu.(2011).Succinate production from different carbon sources under anaerobic conditions by metabolic engineered Escherichia coli strains.METABOLIC ENGINEERING,13(3),328-335. |
| MLA | Wang, Jian,et al."Succinate production from different carbon sources under anaerobic conditions by metabolic engineered Escherichia coli strains".METABOLIC ENGINEERING 13.3(2011):328-335. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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