Extraction and characterization of soil hydrogenases oxidizing atmospheric hydrogen
文献类型：期刊论文
| 作者 | Guo, Rongbo1,2 ; Conrad, Ralf1
|
| 刊名 | SOIL BIOLOGY & BIOCHEMISTRY
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 40期号:5页码:1149-1154 |
| 关键词 | hydrogen consumption tritium exchange abiontic soil enzyme kinetic parameter pH optimum temperature optimum |
| 中文摘要 | Soils are the largest sink of atmospheric hydrogen contributing about 75% to the total budget. Atmospheric H, is assumed to be oxidized in soil by abiontic soil hydrogenases. Extraction of a forest soil with a slightly alkaline (pH 8.5) buffer containing polyethylene glycol (PEG), followed by filtration yielded a bacteria-free extract that oxidized H-2 at ambient concentrations (0.2-2.0 ppmv). Hydrogenase activity was assayed by gas chromatographic analysis of H-2 consumption and by conversion of H-3(2) to tritiated water. Only less than 2% of the original activity was recovered in the extract. Kinetic analysis nevertheless resulted in a biphasic kinetics exhibiting two K-m and V-max values that were similar to those detected in the original soil. In addition, activities of both original soil and soil extract showed similar optima at pH 4-6 and at 30 degrees C, indicating that representative fractions of soil hydrogenases were recovered in the extract. Precipitation with PEG or ultrafiltration allowed further purification of the activity, albeit only about 20% of that in the crude extract could be recovered in the precipitate or the fraction > 100 kDa. |
| 英文摘要 | Soils are the largest sink of atmospheric hydrogen contributing about 75% to the total budget. Atmospheric H, is assumed to be oxidized in soil by abiontic soil hydrogenases. Extraction of a forest soil with a slightly alkaline (pH 8.5) buffer containing polyethylene glycol (PEG), followed by filtration yielded a bacteria-free extract that oxidized H-2 at ambient concentrations (0.2-2.0 ppmv). Hydrogenase activity was assayed by gas chromatographic analysis of H-2 consumption and by conversion of H-3(2) to tritiated water. Only less than 2% of the original activity was recovered in the extract. Kinetic analysis nevertheless resulted in a biphasic kinetics exhibiting two K-m and V-max values that were similar to those detected in the original soil. In addition, activities of both original soil and soil extract showed similar optima at pH 4-6 and at 30 degrees C, indicating that representative fractions of soil hydrogenases were recovered in the extract. Precipitation with PEG or ultrafiltration allowed further purification of the activity, albeit only about 20% of that in the crude extract could be recovered in the precipitate or the fraction > 100 kDa. (C) 2007 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | 生物制氢与沼气 |
| 类目[WOS] | Soil Science |
| 研究领域[WOS] | Agriculture |
| 关键词[WOS] | H-2 ; OXIDATION ; MICROORGANISMS ; TEMPERATURE ; BACTERIA ; MOISTURE ; ECONOMY ; METHANE ; PH ; CO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000255492600015 |
| 公开日期 | 2011-09-09 |
| 源URL | [http://ir.qibebt.ac.cn//handle/337004/490]  |
| 专题 | 青岛生物能源与过程研究所_生物制氢与沼气团队 |
| 作者单位 | 1.Max Planck Inst Terr Microbiol, D-35043 Marburg, Germany 2.Chinese Acad Sci, Qingdao Inst Biomass Energy & Bioproc Technol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Rongbo,Conrad, Ralf. Extraction and characterization of soil hydrogenases oxidizing atmospheric hydrogen[J]. SOIL BIOLOGY & BIOCHEMISTRY,2008,40(5):1149-1154. |
| APA | Guo, Rongbo,&Conrad, Ralf.(2008).Extraction and characterization of soil hydrogenases oxidizing atmospheric hydrogen.SOIL BIOLOGY & BIOCHEMISTRY,40(5),1149-1154. |
| MLA | Guo, Rongbo,et al."Extraction and characterization of soil hydrogenases oxidizing atmospheric hydrogen".SOIL BIOLOGY & BIOCHEMISTRY 40.5(2008):1149-1154. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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