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Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp. ECU0401
文献类型：期刊论文
| 作者 | Yu-Cai He ; Jian-He Xu ; Jin-Huan Su ; Li Zhou |
| 刊名 | Applied Biochemistry and Biotechnology
 |
| 出版日期 | 2009 |
| 卷号 | 160期号:5页码:1428-1440 |
| 关键词 | Nitrilase Alcaligenes sp. ECU0401 Glycolic acid Glycolonitrile Immobilization |
| 中文摘要 | Alcaligenes sp. ECU0401 has been isolated from soil samples with high nitrilase activity against glycolonitrile using the enrichment culture technique. The preferred carbon/nitrogen sources and metal ions were sodium acetate, a composite of peptone and yeast extract, and Cu(2+), respectively. Glycolic acid was obtained in a yield of 96.5% after 14 h of biotransformation from a total of 200 mM glycolonitrile in the mode of sequential addition during the cultivation of Alcaligenes sp. ECU0401 in a 5-L jar fermenter. Fifty micromolars of glycolonitrile could be hydrolyzed in a yield of 94.1% by resting cells after 36 h. The microbial nitrilase system could hydrolyze various nitriles with high activity, and no amidase activity and glycolic acid were observed in hydrolyzing glycolamide. It significantly exhibited high enantioselectivity in the hydrolysis of mandelonitrile and 2-chloromandelonitrile (> 99.9% e.e. (p) ). Efficient biocatalyst recycling was achieved as a result of immobilization in glutaraldehyde/polyethylenimine cross-linked carrageenan with immobilized cells exhibiting a biocatalyst productivity of 1,042.2 g glycolic acid per gram dry cell weight after 29 batch recycles. |
| 学科主题 | 生物基化学品 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2011-09-09 |
| 源URL | [http://ir.qibebt.ac.cn//handle/337004/444]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 推荐引用方式 GB/T 7714 | Yu-Cai He,Jian-He Xu,Jin-Huan Su,et al. Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp. ECU0401[J]. Applied Biochemistry and Biotechnology,2009,160(5):1428-1440. |
| APA | Yu-Cai He,Jian-He Xu,Jin-Huan Su,&Li Zhou.(2009).Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp. ECU0401.Applied Biochemistry and Biotechnology,160(5),1428-1440. |
| MLA | Yu-Cai He,et al."Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp. ECU0401".Applied Biochemistry and Biotechnology 160.5(2009):1428-1440. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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