In situ extraction of intracellular l-asparaginase using thermoseparating aqueous two-phase systems
文献类型：期刊论文
| 作者 | Jian-Hang Zhu ; Xi-Luan Yan ; Hong-Jun Chen ; Zhi-Hui Wang |
| 刊名 | Journal of Chromatography A
 |
| 出版日期 | 2007 |
| 卷号 | 1147期号:1页码:127–134 |
| 关键词 | Aqueous two-phase extraction system In situ extraction Cell disruption Intracellular protein Process integration |
| 中文摘要 | The feasibility and generic applicability of directly integrating conventional discrete operations of cell disruption by high pressure homogenizer and the product capture by aqueous two-phase extraction (ATPE) system have been demonstrated for the extraction of intracellular L-asparaginase from E. coli. In a side-by-side comparison with the conventional ATPE process, including cell disruption, centrifugal clarification and following ATPE, purification of L-asparaginase via this novel in situ ATPE process yielded a product of L-asparaginase with a higher specific activity of 94.8 U/(mg protein) and a higher yield of 73.3%, both of which in the conventional ATPE process were 78.6 U/(mg protein) and 52.1 %, respectively. In the purification of L-asparaginase (pI=4.9), product-debris interactions commonly diminish its recovery. It was demonstrated that immediate extraction of L-asparaginase in ATPE systems when it is released at pH 5.0 during cell disruption effectively increased its recovery in the top phase due to the reduced interaction between L-asparaginase and cell debris and the reduced degradation by contaminated protease. In addition, no clarification step and/or disruptate storage are required in this in situ ATPE, which reduced the number of unit operations and thus shortened the overall process time. This novel process has a good potential for the separation of other intracellular biological products. |
| 学科主题 | 生物基化学品 |
| 语种 | 英语 |
| 公开日期 | 2011-09-09 |
| 源URL | [http://ir.qibebt.ac.cn//handle/337004/454]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 推荐引用方式 GB/T 7714 | Jian-Hang Zhu,Xi-Luan Yan,Hong-Jun Chen,et al. In situ extraction of intracellular l-asparaginase using thermoseparating aqueous two-phase systems[J]. Journal of Chromatography A,2007,1147(1):127–134. |
| APA | Jian-Hang Zhu,Xi-Luan Yan,Hong-Jun Chen,&Zhi-Hui Wang.(2007).In situ extraction of intracellular l-asparaginase using thermoseparating aqueous two-phase systems.Journal of Chromatography A,1147(1),127–134. |
| MLA | Jian-Hang Zhu,et al."In situ extraction of intracellular l-asparaginase using thermoseparating aqueous two-phase systems".Journal of Chromatography A 1147.1(2007):127–134. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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