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Improved phloroglucinol production by metabolically engineered Escherichia coli

文献类型：期刊论文

作者Cao, Yujin1,2; Jiang, Xinglin1,2; Zhang, Rubing1; Xian, Mo1
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版日期2011-09-01
卷号91期号:6页码:1545-1552
关键词Acetyl-CoA carboxylase marA Metabolically engineered Escherichia coli phlD Phloroglucinol
ISSN号1432-0614
中文摘要Phloroglucinol is a valuable chemical which has been successfully produced by metabolically engineered Escherichia coli. However, the low productivity remains a bottleneck for large-scale application and cost-effective production. In the present work, we cloned the key biosynthetic gene, phlD (a type III polyketide synthase), into a bacterial expression vector to produce phloroglucinol in E. coli and developed different strategies to re-engineer the recombinant strain for robust synthesis of phloroglucinol. Overexpression of E. coli marA (multiple antibiotic resistance) gene enhanced phloroglucinol resistance and elevated phloroglucinol production to 0.27g/g dry cell weight. Augmentation of the intracellular malonyl coenzyme A (malonyl-CoA) level through coordinated expression of four acetyl-CoA carboxylase (ACCase) subunits increased phloroglucinol production to around 0.27g/g dry cell weight. Furthermore, the coexpression of ACCase and marA caused another marked improvement in phloroglucinol production 0.45g/g dry cell weight, that is, 3.3-fold to the original strain. Under fed-batch conditions, this finally engineered strain accumulated phloroglucinol up to 3.8g/L in the culture 12h after induction, corresponding to a volumetric productivity of 0.32g/L/h. This result was the highest phloroglucinol production to date and showed promising to make the bioprocess economically feasible.
英文摘要Phloroglucinol is a valuable chemical which has been successfully produced by metabolically engineered Escherichia coli. However, the low productivity remains a bottleneck for large-scale application and cost-effective production. In the present work, we cloned the key biosynthetic gene, phlD (a type III polyketide synthase), into a bacterial expression vector to produce phloroglucinol in E. coli and developed different strategies to re-engineer the recombinant strain for robust synthesis of phloroglucinol. Overexpression of E. coli marA (multiple antibiotic resistance) gene enhanced phloroglucinol resistance and elevated phloroglucinol production to 0.27 g/g dry cell weight. Augmentation of the intracellular malonyl coenzyme A (malonyl-CoA) level through coordinated expression of four acetyl-CoA carboxylase (ACCase) subunits increased phloroglucinol production to around 0.27 g/g dry cell weight. Furthermore, the coexpression of ACCase and marA caused another marked improvement in phloroglucinol production 0.45 g/g dry cell weight, that is, 3.3-fold to the original strain. Under fed-batch conditions, this finally engineered strain accumulated phloroglucinol up to 3.8 g/L in the culture 12 h after induction, corresponding to a volumetric productivity of 0.32 g/L/h. This result was the highest phloroglucinol production to date and showed promising to make the bioprocess economically feasible.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]2,4-DIACETYLPHLOROGLUCINOL-PRODUCING FLUORESCENT PSEUDOMONAS ; MULTIPLE ANTIBIOTIC-RESISTANCE ; ACETYL-COA ; GENE-CLUSTER ; MALONYL-COA ; BIOSYNTHESIS ; REGULATOR ; PHLD ; DIVERSITY ; LOCUS
收录类别SCI
语种英语
WOS记录号WOS:000294214900008
公开日期2011-09-13
源URL[http://ir.qibebt.ac.cn//handle/337004/676]  
专题青岛生物能源与过程研究所_材料生物技术研究中心
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Cao, Yujin,Jiang, Xinglin,Zhang, Rubing,et al. Improved phloroglucinol production by metabolically engineered Escherichia coli[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,91(6):1545-1552.
APA Cao, Yujin,Jiang, Xinglin,Zhang, Rubing,&Xian, Mo.(2011).Improved phloroglucinol production by metabolically engineered Escherichia coli.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,91(6),1545-1552.
MLA Cao, Yujin,et al."Improved phloroglucinol production by metabolically engineered Escherichia coli".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 91.6(2011):1545-1552.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

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