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Morphological regulation of Aspergillus niger to improve citric acid production by chsC gene silencing

文献类型：期刊论文

作者Sun, Xiaowen1,2; Wu, Hefang1; Zhao, Genhai1; Li, Zhemin1; Wu, Xihua1; Liu, Hui1; Zheng, Zhiming1
刊名BIOPROCESS AND BIOSYSTEMS ENGINEERING
出版日期2018-07-01
卷号41期号:7页码:1029-1038
关键词Aspergillus niger Morphology Citric acid Chitin synthase RNA interference
ISSN号1615-7591
DOI10.1007/s00449-018-1932-1
通讯作者Liu, Hui(lhazrael@126.com) ; Zheng, Zhiming(zhengzhiming2014@163.com)
英文摘要The mycelial morphology of Aspergillus niger, a major filamentous fungus used for citric acid production, is important for citric acid synthesis during submerged fermentation. To investigate the involvement of the chitin synthase gene, chsC, in morphogenesis and citric acid production in A. niger, an RNAi system was constructed to silence chsC and the morphological mutants were screened after transformation. The compactness of the mycelial pellets was obviously reduced in the morphological mutants, with lower proportion of dispersed mycelia. These morphological changes have caused a decrease in viscosity and subsequent improvement in oxygen and mass transfer efficiency, which may be conducive for citric acid accumulation. All the transformants exhibited improvements in citric acid production; in particular, chsC-3 showed 42.6% higher production than the original strain in the shake flask. Moreover, the high-yield strain chsC-3 exhibited excellent citric acid production potential in the scale-up process.The citric acid yield and the conversion rate of glucose of chsC-3 were both improved by 3.6%, when compared with that of the original strain in the stirred tank bioreactor.
WOS关键词CHITIN SYNTHASE GENES ; IMPROVED ENZYME-PRODUCTION ; PENICILLIUM-CHRYSOGENUM ; FILAMENTOUS FUNGI ; IMAGE-ANALYSIS ; FED-BATCH ; CLASS-I ; NIDULANS ; ORYZAE ; GROWTH
资助项目National High-Tech Research and Development Program of China[2014AA021704] ; Natural Science Foundation of Anhui Province[1608085QC46] ; Major Projects of Science and Technology in Anhui Province[17030801036]
WOS研究方向Biotechnology & Applied Microbiology ; Engineering
语种英语
WOS记录号WOS:000435942800011
出版者SPRINGER
资助机构National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Natural Science Foundation of Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; Major Projects of Science and Technology in Anhui Province ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; National High-Tech Research and Development Program of China ; 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源URL[http://ir.hfcas.ac.cn:8080/handle/334002/37245]  
专题合肥物质科学研究院_中科院强磁场科学中心
合肥物质科学研究院_技术生物与农业工程研究所
通讯作者Liu, Hui; Zheng, Zhiming
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Bioengn, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Xiaowen,Wu, Hefang,Zhao, Genhai,et al. Morphological regulation of Aspergillus niger to improve citric acid production by chsC gene silencing[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2018,41(7):1029-1038.
APA Sun, Xiaowen.,Wu, Hefang.,Zhao, Genhai.,Li, Zhemin.,Wu, Xihua.,...&Zheng, Zhiming.(2018).Morphological regulation of Aspergillus niger to improve citric acid production by chsC gene silencing.BIOPROCESS AND BIOSYSTEMS ENGINEERING,41(7),1029-1038.
MLA Sun, Xiaowen,et al."Morphological regulation of Aspergillus niger to improve citric acid production by chsC gene silencing".BIOPROCESS AND BIOSYSTEMS ENGINEERING 41.7(2018):1029-1038.

入库方式： OAI收割

来源：合肥物质科学研究院

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