A microfluidic surface-enhanced Raman spectroscopy approach for assessing the particle number effect of AgNPs on cytotoxicity
文献类型：期刊论文
| 作者 | Zhai, Zhimin1,5; Nie, Mengyue2; Guan, Yong3; Zhang, Fengqiu1,3,4; Chen, Liang3 ; Muhammad1,5; Du, Wenbin2; Liu, Gang3; Tian, Yangchao3; Huang, Qing1,3,5
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| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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| 出版日期 | 2018-10-30 |
| 卷号 | 162页码:529-535 |
| 关键词 | Silver nanoparticles (Ag NPs) Cytotoxicity Surface enhanced Raman spectroscopy (SERS) HeLa cells Microfluidic device |
| ISSN号 | 0147-6513 |
| DOI | 10.1016/j.ecoenv.2018.07.026 |
| 通讯作者 | Huang, Qing(huangq@ipp.ac.cn) |
| 英文摘要 | Silver nanoparticles (Ag NPs) have well-known antibacterial properties and are widely applied in various medical products and general commodities. Although many studies have addressed the toxicity of Ag NPs to mammalian cells, the direct relationship between the number of Ag NPs in living cells and the corresponding cell toxicity has not yet been explicitly demonstrated. In this work, a simple and reusable microfluidic device composed of a quartz cover slip and a glass plate with etched micro-channel and micro-wells was employed for separating and trapping single living cells. The device was silanized to render the surface hydrophobic. For simplicity, HeLa cells as the model cancer cells were used in the study, which were pipette-loaded into an array of micro wells based on dead-end filling. Surface enhanced Raman spectroscopy (SERS) was then employed to examine the living cancer cells and assessed number and distribution of Ag NPs in the cells. Combined with the cell viability assay, we therefore correlated the number of Ag NPs in the cell with the toxicity to the cell directly. |
| WOS关键词 | SILVER NANOPARTICLES ; CANCER-CELLS ; FOLIC-ACID ; SCATTERING SPECTROSCOPY ; DEPENDENT TOXICITY ; OXIDATIVE STRESS ; SERS ; NANOMATERIALS ; FLUORESCENCE ; HEALTH |
| 资助项目 | National Basic Research Program of China[2014CB932002] ; National Natural Science Foundation of China[11635013] ; National Natural Science Foundation of China[11475217] ; National Natural Science Foundation of China[11775272] |
| WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000440879500062 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 资助机构 | National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/37688]  |
| 专题 | 合肥物质科学研究院_技术生物与农业工程研究所 |
| 通讯作者 | Huang, Qing |
| 作者单位 | 1.Chinese Acad Sci, Inst Tech Biol & Agr Engn, Key Lab High Magnet Field & IonBeam Phys Biol,Hef, Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230031, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 3.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230026, Anhui, Peoples R China 4.Zhengzhou Univ, Sch Phys Engn, Zhengzhou, Henan, Peoples R China 5.Univ Sci & Technol China, Univ Chinese Acad Sci, Hefei, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhai, Zhimin,Nie, Mengyue,Guan, Yong,et al. A microfluidic surface-enhanced Raman spectroscopy approach for assessing the particle number effect of AgNPs on cytotoxicity[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2018,162:529-535. |
| APA | Zhai, Zhimin.,Nie, Mengyue.,Guan, Yong.,Zhang, Fengqiu.,Chen, Liang.,...&Huang, Qing.(2018).A microfluidic surface-enhanced Raman spectroscopy approach for assessing the particle number effect of AgNPs on cytotoxicity.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,162,529-535. |
| MLA | Zhai, Zhimin,et al."A microfluidic surface-enhanced Raman spectroscopy approach for assessing the particle number effect of AgNPs on cytotoxicity".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 162(2018):529-535. |
入库方式： OAI收割
来源：合肥物质科学研究院
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