Study on flow characteristics of solid/liquid system in lysozyme crystal growth
文献类型：期刊论文
| 作者 | Cui HL(崔海亮) ; Yu Y(于泳); Chen WC(陈万春); Kang Q(康奇)
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| 刊名 | Chinese Science Bulletin/中国科学通报(英文版)
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| 出版日期 | 2007 |
| 卷号 | 52期号:9页码:1196-1204 |
| 通讯作者邮箱 | yuyong@imech.ac.cn |
| 关键词 | Protein Crystal Batch Method Buoyancy-Driven Flows Particle Image Velocimetry Shear Force Protein Crystal Kinetics Crystallization Microgravity Convection Transport Virus |
| ISSN号 | 1001-6538 |
| 通讯作者 | Yu, Y (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China. |
| 中文摘要 | During the process of lysozyme protein crystallization with batch method, the macroscopic flow field of solid/liquid system was observed by particle image velocimetry (PIV). Furthermore, a normal growth rate of (110) face and local flow field around a single protein crystal were obtained by a long work distance microscope. The experimental results showed that the average velocity, the maximal velocity of macroscopic solid/liquid system and the velocity of local flow field around single protein crystal were fluctuant. The effective boundary layer thickness delta(eff), the concentration at the interface Q and the characteristic velocity V were calculated using a convection-diffusion model. The results showed that the growth of lysozyme crystal in this experiment was dominated by interfacial kinetics rather than bulk transport, and the function of buoyancy-driven flow in bulk transport was small, however, the effect of bulk transport in crystal growth had a tendency to increase with the increase of lysozyme concentration. The calculated results, also showed that the order of magnitude of shear force was about 10(-21) N, which was much less than the bond force between the lysozyme molecules. Therefore the shear force induced by buoyancy-driven flows cannot remove the protein molecules from the interface of crystal. |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | PROTEIN CRYSTAL ; KINETICS ; CRYSTALLIZATION ; MICROGRAVITY ; CONVECTION ; TRANSPORT ; VIRUS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247545800008 |
| 公开日期 | 2009-08-03 ; 2010-06-13 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/33951]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Cui HL,Yu Y,Chen WC,et al. Study on flow characteristics of solid/liquid system in lysozyme crystal growth[J]. Chinese Science Bulletin/中国科学通报(英文版),2007,52(9):1196-1204. |
| APA | 崔海亮,于泳,陈万春,&康奇.(2007).Study on flow characteristics of solid/liquid system in lysozyme crystal growth.Chinese Science Bulletin/中国科学通报(英文版),52(9),1196-1204. |
| MLA | 崔海亮,et al."Study on flow characteristics of solid/liquid system in lysozyme crystal growth".Chinese Science Bulletin/中国科学通报(英文版) 52.9(2007):1196-1204. |
入库方式： OAI收割
来源：力学研究所
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