中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Callus induction and high-frequency plant regeneration from hypocotyl and cotyledon explants of Arctium lappa L.

文献类型:期刊论文

作者He, W. T.; Hou, S. W.; Wang, C. Y.
刊名IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
出版日期2006-09-01
卷号42页码:411-414
关键词Arctium lappa L callus induction organogenesis plant regeneration somatic embryogenesis
ISSN号1054-5476
DOI10.1079/IVP2006801
英文摘要This study describes a protocol for plant regeneration from cultured seedling explants of Arctium lappa. Hypocotyls and cotyledons of A. lappa were induced to form callus by culturing on Murashige and Skoog (MS) medium supplemented with 2.0 mg l(-1) 2,4-dichlorophenoxyacetic acid and 0.5-2.0 mg l(-1) benzyladenine (BA). Formation of adventitious buds could be induced from calluses or explants directly by culturing on MS medium containing 1.0-2.0 mg l(-1) alpha-naphthaleneacetic acid (NAA) and 0.5-2.0 mg l(-1) BA. These regenerated shoots were rooted on MS medium with 1.0 mg l(-1) indole-3-butyric acid or indole-3-acetic acid in combination with 1.0 mg l(-1) NAA. The regenerated plants acclimatized in soil were normal morphologically and in growth characters. They flowered and set seeds in the following year after acclimatization.
WOS关键词LIGNANS ; RATS
WOS研究方向Plant Sciences ; Cell Biology ; Developmental Biology
语种英语
WOS记录号WOS:000242217900008
出版者CABI PUBLISHING
源URL[http://119.78.100.186/handle/113462/25998]  
专题中国科学院近代物理研究所
通讯作者Hou, S. W.
作者单位1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714
He, W. T.,Hou, S. W.,Wang, C. Y.. Callus induction and high-frequency plant regeneration from hypocotyl and cotyledon explants of Arctium lappa L.[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2006,42:411-414.
APA He, W. T.,Hou, S. W.,&Wang, C. Y..(2006).Callus induction and high-frequency plant regeneration from hypocotyl and cotyledon explants of Arctium lappa L..IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,42,411-414.
MLA He, W. T.,et al."Callus induction and high-frequency plant regeneration from hypocotyl and cotyledon explants of Arctium lappa L.".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 42(2006):411-414.

入库方式: OAI收割

来源:近代物理研究所

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